Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices

Josiane P. Lafleur, Radoslaw Kwapiszewski, Thomas Glasdam Jensen, Jörg Peter Kutter

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

    Abstract

    The ability to immobilize biomolecules at specific locations on the surface of solid supports is central to many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in thiol-ene microfluidic chips. Adjusting the stoichiometric ratio of “thiol” and “ene” monomers present in the microfluidic chip bulk material provides a simple and efficient way of tuning the chip’s surface chemistry. Here, thiol-ene chips displaying an excess of functional thiol groups at their surfaces are functionalized with biotin and streptavidin in a controlled fashion using photolithography. We also present quantitative data on the number of functional groups available for surface modification on thiol-ene substrates and their stability.
    Original languageEnglish
    Title of host publicationProceedings of MicroTAS 2012
    Publication date2012
    Pages1258-1260
    Publication statusPublished - 2012
    Event16th International Conference of Miniaturized Systems for Chemistry and Life Sciences - Okinawa Convention Center, Okinawa, Japan
    Duration: 28 Oct 20121 Nov 2012
    Conference number: 16
    http://www.microtas12.org/

    Conference

    Conference16th International Conference of Miniaturized Systems for Chemistry and Life Sciences
    Number16
    LocationOkinawa Convention Center
    CountryJapan
    CityOkinawa
    Period28/10/201201/11/2012
    Internet address

    Cite this

    Lafleur, J. P., Kwapiszewski, R., Jensen, T. G., & Kutter, J. P. (2012). Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices. In Proceedings of MicroTAS 2012 (pp. 1258-1260)