Rapid detection and identification of Stachybotrys and Chaetomium species using tissue PCR analysis

Anna Malgorzata Lewinska, Ruut Hannele Peuhkuri, Carsten Rode, Birgitte Andersen, Jakob Blæsbjerg Hoof

Research output: Contribution to journalJournal articleResearchpeer-review


Indoor fungi are a worldwide problem causing negative health effects for infected building's occupants and even deterioration of building structures. Different fungal species affect buildings and their inhabitants differently. Therefore, rapid and accurate identification of fungi to the species level is essential for health risk assessment and building remediation. This study focuses on molecular identification of two common indoor fungal genera: Stachybotrys and Chaetomium. This study proposes two new DNA barcode candidates for Stachybotrys and Chaetomium: the gene encoding mitogen activated protein kinase (hogA) and the intergenic region between histone 3 and histone 4 (h3-h4) as well as it introduces a rapid - 3.5 h - protocol for direct Stachybotrys and Chaetomium species identification, which bypasses culture cultivation, DNA extraction and DNA sequencing.
Original languageEnglish
JournalJournal of Microbiological Methods
Pages (from-to)115-122
Number of pages8
Publication statusPublished - 2016


  • DNA barcoding
  • Indoor fungi
  • ITS
  • PCR
  • Species identification

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