Quorum Sensing Regulation in Aeromonas hydrophila

Christian Garde, Thomas Bjarnsholt, Michael Givskov, Tim Holm Jakobsen, Morten Hentzer, Anetta Claussen, Kim Sneppen, Jesper Ferkinghoff-Borg, Thomas Sams

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We present detailed results on the C4-HSL-mediated quorum sensing (QS) regulatory system of the opportunistic Gram-negative bacterium Aeromonas hydrophila. This bacterium contains a particularly simple QS system that allows for a detailed modeling of kinetics. In a model system (i.e., the Escherichia coli monitor strain MH205), the C4-HSL production of A. hydrophila is interrupted by fusion of gfp(ASV). In the present in vitro study, we measure the response of the QS regulatory ahyRI locus in the monitor strain to predetermined concentrations of C4-HSL signal molecules. A minimal kinetic model describes the data well. It can be solved analytically, providing substantial insight into the QS mechanism: at high concentrations of signal molecules, a slow decay of the activated regulator sets the timescale for the QS regulation loop. Slow saturation ensures that, in an A. hydrophila cell, the QS system is activated only by signal molecules produced by other A. hydrophila cells. Separate information on the ahyR and ahyI loci can be extracted, thus allowing the probe to be used in identifying the target when testing QS inhibitors.
Original languageEnglish
JournalJournal of Molecular Biology
Volume396
Issue number4
Pages (from-to)849-857
ISSN0022-2836
DOIs
Publication statusPublished - 2010

Cite this

Garde, C., Bjarnsholt, T., Givskov, M., Jakobsen, T. H., Hentzer, M., Claussen, A., ... Sams, T. (2010). Quorum Sensing Regulation in Aeromonas hydrophila. Journal of Molecular Biology, 396(4), 849-857. https://doi.org/10.1016/j.jmb.2010.01.002
Garde, Christian ; Bjarnsholt, Thomas ; Givskov, Michael ; Jakobsen, Tim Holm ; Hentzer, Morten ; Claussen, Anetta ; Sneppen, Kim ; Ferkinghoff-Borg, Jesper ; Sams, Thomas. / Quorum Sensing Regulation in Aeromonas hydrophila. In: Journal of Molecular Biology. 2010 ; Vol. 396, No. 4. pp. 849-857.
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title = "Quorum Sensing Regulation in Aeromonas hydrophila",
abstract = "We present detailed results on the C4-HSL-mediated quorum sensing (QS) regulatory system of the opportunistic Gram-negative bacterium Aeromonas hydrophila. This bacterium contains a particularly simple QS system that allows for a detailed modeling of kinetics. In a model system (i.e., the Escherichia coli monitor strain MH205), the C4-HSL production of A. hydrophila is interrupted by fusion of gfp(ASV). In the present in vitro study, we measure the response of the QS regulatory ahyRI locus in the monitor strain to predetermined concentrations of C4-HSL signal molecules. A minimal kinetic model describes the data well. It can be solved analytically, providing substantial insight into the QS mechanism: at high concentrations of signal molecules, a slow decay of the activated regulator sets the timescale for the QS regulation loop. Slow saturation ensures that, in an A. hydrophila cell, the QS system is activated only by signal molecules produced by other A. hydrophila cells. Separate information on the ahyR and ahyI loci can be extracted, thus allowing the probe to be used in identifying the target when testing QS inhibitors.",
author = "Christian Garde and Thomas Bjarnsholt and Michael Givskov and Jakobsen, {Tim Holm} and Morten Hentzer and Anetta Claussen and Kim Sneppen and Jesper Ferkinghoff-Borg and Thomas Sams",
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Garde, C, Bjarnsholt, T, Givskov, M, Jakobsen, TH, Hentzer, M, Claussen, A, Sneppen, K, Ferkinghoff-Borg, J & Sams, T 2010, 'Quorum Sensing Regulation in Aeromonas hydrophila', Journal of Molecular Biology, vol. 396, no. 4, pp. 849-857. https://doi.org/10.1016/j.jmb.2010.01.002

Quorum Sensing Regulation in Aeromonas hydrophila. / Garde, Christian; Bjarnsholt, Thomas; Givskov, Michael; Jakobsen, Tim Holm; Hentzer, Morten; Claussen, Anetta; Sneppen, Kim; Ferkinghoff-Borg, Jesper; Sams, Thomas.

In: Journal of Molecular Biology, Vol. 396, No. 4, 2010, p. 849-857.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Quorum Sensing Regulation in Aeromonas hydrophila

AU - Garde, Christian

AU - Bjarnsholt, Thomas

AU - Givskov, Michael

AU - Jakobsen, Tim Holm

AU - Hentzer, Morten

AU - Claussen, Anetta

AU - Sneppen, Kim

AU - Ferkinghoff-Borg, Jesper

AU - Sams, Thomas

PY - 2010

Y1 - 2010

N2 - We present detailed results on the C4-HSL-mediated quorum sensing (QS) regulatory system of the opportunistic Gram-negative bacterium Aeromonas hydrophila. This bacterium contains a particularly simple QS system that allows for a detailed modeling of kinetics. In a model system (i.e., the Escherichia coli monitor strain MH205), the C4-HSL production of A. hydrophila is interrupted by fusion of gfp(ASV). In the present in vitro study, we measure the response of the QS regulatory ahyRI locus in the monitor strain to predetermined concentrations of C4-HSL signal molecules. A minimal kinetic model describes the data well. It can be solved analytically, providing substantial insight into the QS mechanism: at high concentrations of signal molecules, a slow decay of the activated regulator sets the timescale for the QS regulation loop. Slow saturation ensures that, in an A. hydrophila cell, the QS system is activated only by signal molecules produced by other A. hydrophila cells. Separate information on the ahyR and ahyI loci can be extracted, thus allowing the probe to be used in identifying the target when testing QS inhibitors.

AB - We present detailed results on the C4-HSL-mediated quorum sensing (QS) regulatory system of the opportunistic Gram-negative bacterium Aeromonas hydrophila. This bacterium contains a particularly simple QS system that allows for a detailed modeling of kinetics. In a model system (i.e., the Escherichia coli monitor strain MH205), the C4-HSL production of A. hydrophila is interrupted by fusion of gfp(ASV). In the present in vitro study, we measure the response of the QS regulatory ahyRI locus in the monitor strain to predetermined concentrations of C4-HSL signal molecules. A minimal kinetic model describes the data well. It can be solved analytically, providing substantial insight into the QS mechanism: at high concentrations of signal molecules, a slow decay of the activated regulator sets the timescale for the QS regulation loop. Slow saturation ensures that, in an A. hydrophila cell, the QS system is activated only by signal molecules produced by other A. hydrophila cells. Separate information on the ahyR and ahyI loci can be extracted, thus allowing the probe to be used in identifying the target when testing QS inhibitors.

U2 - 10.1016/j.jmb.2010.01.002

DO - 10.1016/j.jmb.2010.01.002

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JF - Journal of Molecular Biology

SN - 0022-2836

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Garde C, Bjarnsholt T, Givskov M, Jakobsen TH, Hentzer M, Claussen A et al. Quorum Sensing Regulation in Aeromonas hydrophila. Journal of Molecular Biology. 2010;396(4):849-857. https://doi.org/10.1016/j.jmb.2010.01.002