Quorum-sensing-directed protein expression in Serratia proteamaculans B5a

Allan Beck Christensen, Kathrin Riedel, Leo Eberl, Lars Flodgaard, Søren Molin, Lone Gram, Michael Christian Givskov

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

N-Acyl-L-homoserine-lactone-producing Serratia species are frequently encountered in spoiling foods of vegetable and protein origin. The role of quorum sensing in the food spoiling properties of these bacteria is currently being investigated. A set of luxR luxI homologous genes encoding a putative quorum sensor was identified in the N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL)-producing Serratia proteamaculans strain B5a. The 3-oxo-C6-HSL synthase SprI showed 79% similarity with Esal from Pantoea stewartii and the putative regulatory protein SprR was 86% similar to the SpnR of Serratia marcescens. Proteome analysis suggested that the presence of at least 39 intracellular proteins was affected by the 3-oxo-C6-HSL-based quorum sensing system. The lipB-encoded secretion system was identified as one target gene of the quorum sensing system. LipB was required for the production of extracellular lipolytic and proteolytic activities, thus rendering the production of food-deterioration-relevant exoenzymes indirectly under the control of quorum sensing. Strain B5a caused quorum-sensing-controlled spoilage of milk. Furthermore, chitinolytic activity was controlled by quorum sensing. This control appeared to be direct and not mediated via LipB. The data presented here demonstrate that quorum-sensing-controlled exoenzymic activities affect food quality.
Original languageEnglish
JournalMicrobiology
Volume149
Issue number2
Pages (from-to)471-483
ISSN1350-0872
Publication statusPublished - 2003

Keywords

  • analysis

Cite this

Christensen, A. B., Riedel, K., Eberl, L., Flodgaard, L., Molin, S., Gram, L., & Givskov, M. C. (2003). Quorum-sensing-directed protein expression in Serratia proteamaculans B5a. Microbiology, 149(2), 471-483.
Christensen, Allan Beck ; Riedel, Kathrin ; Eberl, Leo ; Flodgaard, Lars ; Molin, Søren ; Gram, Lone ; Givskov, Michael Christian. / Quorum-sensing-directed protein expression in Serratia proteamaculans B5a. In: Microbiology. 2003 ; Vol. 149, No. 2. pp. 471-483.
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abstract = "N-Acyl-L-homoserine-lactone-producing Serratia species are frequently encountered in spoiling foods of vegetable and protein origin. The role of quorum sensing in the food spoiling properties of these bacteria is currently being investigated. A set of luxR luxI homologous genes encoding a putative quorum sensor was identified in the N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL)-producing Serratia proteamaculans strain B5a. The 3-oxo-C6-HSL synthase SprI showed 79{\%} similarity with Esal from Pantoea stewartii and the putative regulatory protein SprR was 86{\%} similar to the SpnR of Serratia marcescens. Proteome analysis suggested that the presence of at least 39 intracellular proteins was affected by the 3-oxo-C6-HSL-based quorum sensing system. The lipB-encoded secretion system was identified as one target gene of the quorum sensing system. LipB was required for the production of extracellular lipolytic and proteolytic activities, thus rendering the production of food-deterioration-relevant exoenzymes indirectly under the control of quorum sensing. Strain B5a caused quorum-sensing-controlled spoilage of milk. Furthermore, chitinolytic activity was controlled by quorum sensing. This control appeared to be direct and not mediated via LipB. The data presented here demonstrate that quorum-sensing-controlled exoenzymic activities affect food quality.",
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author = "Christensen, {Allan Beck} and Kathrin Riedel and Leo Eberl and Lars Flodgaard and S{\o}ren Molin and Lone Gram and Givskov, {Michael Christian}",
year = "2003",
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Christensen, AB, Riedel, K, Eberl, L, Flodgaard, L, Molin, S, Gram, L & Givskov, MC 2003, 'Quorum-sensing-directed protein expression in Serratia proteamaculans B5a', Microbiology, vol. 149, no. 2, pp. 471-483.

Quorum-sensing-directed protein expression in Serratia proteamaculans B5a. / Christensen, Allan Beck; Riedel, Kathrin; Eberl, Leo; Flodgaard, Lars; Molin, Søren; Gram, Lone; Givskov, Michael Christian.

In: Microbiology, Vol. 149, No. 2, 2003, p. 471-483.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Quorum-sensing-directed protein expression in Serratia proteamaculans B5a

AU - Christensen, Allan Beck

AU - Riedel, Kathrin

AU - Eberl, Leo

AU - Flodgaard, Lars

AU - Molin, Søren

AU - Gram, Lone

AU - Givskov, Michael Christian

PY - 2003

Y1 - 2003

N2 - N-Acyl-L-homoserine-lactone-producing Serratia species are frequently encountered in spoiling foods of vegetable and protein origin. The role of quorum sensing in the food spoiling properties of these bacteria is currently being investigated. A set of luxR luxI homologous genes encoding a putative quorum sensor was identified in the N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL)-producing Serratia proteamaculans strain B5a. The 3-oxo-C6-HSL synthase SprI showed 79% similarity with Esal from Pantoea stewartii and the putative regulatory protein SprR was 86% similar to the SpnR of Serratia marcescens. Proteome analysis suggested that the presence of at least 39 intracellular proteins was affected by the 3-oxo-C6-HSL-based quorum sensing system. The lipB-encoded secretion system was identified as one target gene of the quorum sensing system. LipB was required for the production of extracellular lipolytic and proteolytic activities, thus rendering the production of food-deterioration-relevant exoenzymes indirectly under the control of quorum sensing. Strain B5a caused quorum-sensing-controlled spoilage of milk. Furthermore, chitinolytic activity was controlled by quorum sensing. This control appeared to be direct and not mediated via LipB. The data presented here demonstrate that quorum-sensing-controlled exoenzymic activities affect food quality.

AB - N-Acyl-L-homoserine-lactone-producing Serratia species are frequently encountered in spoiling foods of vegetable and protein origin. The role of quorum sensing in the food spoiling properties of these bacteria is currently being investigated. A set of luxR luxI homologous genes encoding a putative quorum sensor was identified in the N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL)-producing Serratia proteamaculans strain B5a. The 3-oxo-C6-HSL synthase SprI showed 79% similarity with Esal from Pantoea stewartii and the putative regulatory protein SprR was 86% similar to the SpnR of Serratia marcescens. Proteome analysis suggested that the presence of at least 39 intracellular proteins was affected by the 3-oxo-C6-HSL-based quorum sensing system. The lipB-encoded secretion system was identified as one target gene of the quorum sensing system. LipB was required for the production of extracellular lipolytic and proteolytic activities, thus rendering the production of food-deterioration-relevant exoenzymes indirectly under the control of quorum sensing. Strain B5a caused quorum-sensing-controlled spoilage of milk. Furthermore, chitinolytic activity was controlled by quorum sensing. This control appeared to be direct and not mediated via LipB. The data presented here demonstrate that quorum-sensing-controlled exoenzymic activities affect food quality.

KW - analysis

M3 - Journal article

VL - 149

SP - 471

EP - 483

JO - Microbiology

JF - Microbiology

SN - 1350-0872

IS - 2

ER -

Christensen AB, Riedel K, Eberl L, Flodgaard L, Molin S, Gram L et al. Quorum-sensing-directed protein expression in Serratia proteamaculans B5a. Microbiology. 2003;149(2):471-483.