Quantitative studies of subdiffusion in living cells and actin networks

Emilia-Laura Munteanu (Invited author), Anja Lea Olsen (Invited author), Iva Marija Tolic-Nørrelykke (Invited author), Henrik Flyvbjerg (Invited author), Lene B Oddershede (Invited author), Kirstine Berg-Sørensen (Invited author)

Research output: Contribution to journalConference articleResearchpeer-review

Abstract

Optical tweezers are a versatile tool in biophysics and have matured from a tool of manipulation to a tool of precise measurements. We argue here that the data analysis with advantage can be developed to a level of sophistication that matches that of the instrument. We review methods of analysis of optical tweezers data, primarily baed on the power spectra of time series of postions for trapped spherical objects. The majority of precise studies in the literature are performed on in vitro systems, whereas in the present work, an example of an in vivo system is presented for which precise power spectral analysis is both useful and necessary. The biological system is the cytoplasm of fission yeast, S. pombe, in which we observe subdiffusion of lipid granuli. in a search for the cause of subdiffusion, we chemically disrupt the actin network in the cytoplasm and further consider in vitro networks of filamenteous actin undergoing similar chemical disruption
Original languageEnglish
JournalBiophysical Reviews and Letters
Volume1
Issue number4
Pages (from-to)411-421
ISSN1793-0480
DOIs
Publication statusPublished - 2006
EventBio-Systems - Berlin
Duration: 1 Jan 2006 → …

Conference

ConferenceBio-Systems
CityBerlin
Period01/01/2006 → …

Cite this

Munteanu, E-L., Olsen, A. L., Tolic-Nørrelykke, I. M., Flyvbjerg, H., Oddershede, L. B., & Berg-Sørensen, K. (2006). Quantitative studies of subdiffusion in living cells and actin networks. Biophysical Reviews and Letters, 1(4), 411-421. https://doi.org/10.1142/S1793048006000343