Quantitative Proteomics Analysis of Barley-Based Liquid Feed and the Effect of Protease Inhibitors and NADPH-Dependent Thioredoxin Reductase/Thioredoxin (NTR/Trx) System

Abida Sultan*, Birgit Andersen, Jesper Bjerg Christensen, Hanne Damgaard Poulsen, Birte Svensson, Christine Finnie

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review


Liquid feeding strategies have been devised with the aim of enhancing grain nutrient availability for livestock. It is characterized by a steeping/soaking period that softens the grains and initiates mobilization of seed storage reserves. The present study uses 2D gel-based proteomics to investigate the role of proteolysis and reduction by thioredoxins over a 48 h steeping period by monitoring protein abundance dynamics in barley-based liquid feed samples supplemented with either protease inhibitors or NADPH-dependent thioredoxin reductase/thioredoxin (NTR/Trx). Several full-length storage proteins were only identified in the water-extractable fraction of feed containing protease inhibitors, illustrating significant inhibition of proteolytic activities arising during the steeping period. Application of functional NTR/Trx to liquid feed reductively increased the solubility of known and potentially new Trx-target proteins, e.g., outer membrane protein X, and their susceptibility to proteolysis. Thus, the NTR/Trx system exhibits important potential as a feed additive to enhance nutrient digestibility in monogastric animals.

Original languageEnglish
JournalJournal of Agricultural and Food Chemistry
Issue number22
Pages (from-to)6432-6444
Number of pages13
Publication statusPublished - 2019


  • barley proteome
  • mass spectrometry
  • NADPH-dependent thioredoxin reductase/thioredoxin system
  • protease inhibitors

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