Quantitative Proteomics Analysis of Barley-Based Liquid Feed and the Effect of Protease Inhibitors and NADPH-Dependent Thioredoxin Reductase/Thioredoxin (NTR/Trx) System

Abida Sultan*, Birgit Andersen, Jesper Bjerg Christensen, Hanne Damgaard Poulsen, Birte Svensson, Christine Finnie

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Liquid feeding strategies have been devised with the aim of enhancing grain nutrient availability for livestock. It is characterized by a steeping/soaking period that softens the grains and initiates mobilization of seed storage reserves. The present study uses 2D gel-based proteomics to investigate the role of proteolysis and reduction by thioredoxins over a 48 h steeping period by monitoring protein abundance dynamics in barley-based liquid feed samples supplemented with either protease inhibitors or NADPH-dependent thioredoxin reductase/thioredoxin (NTR/Trx). Several full-length storage proteins were only identified in the water-extractable fraction of feed containing protease inhibitors, illustrating significant inhibition of proteolytic activities arising during the steeping period. Application of functional NTR/Trx to liquid feed reductively increased the solubility of known and potentially new Trx-target proteins, e.g., outer membrane protein X, and their susceptibility to proteolysis. Thus, the NTR/Trx system exhibits important potential as a feed additive to enhance nutrient digestibility in monogastric animals.

Original languageEnglish
JournalJournal of Agricultural and Food Chemistry
Volume67
Issue number22
Pages (from-to)6432-6444
Number of pages13
ISSN0021-8561
DOIs
Publication statusPublished - 2019

Keywords

  • barley proteome
  • mass spectrometry
  • NADPH-dependent thioredoxin reductase/thioredoxin system
  • protease inhibitors

Cite this

@article{867ad3b531964f55b3424164b8078abd,
title = "Quantitative Proteomics Analysis of Barley-Based Liquid Feed and the Effect of Protease Inhibitors and NADPH-Dependent Thioredoxin Reductase/Thioredoxin (NTR/Trx) System",
abstract = "Liquid feeding strategies have been devised with the aim of enhancing grain nutrient availability for livestock. It is characterized by a steeping/soaking period that softens the grains and initiates mobilization of seed storage reserves. The present study uses 2D gel-based proteomics to investigate the role of proteolysis and reduction by thioredoxins over a 48 h steeping period by monitoring protein abundance dynamics in barley-based liquid feed samples supplemented with either protease inhibitors or NADPH-dependent thioredoxin reductase/thioredoxin (NTR/Trx). Several full-length storage proteins were only identified in the water-extractable fraction of feed containing protease inhibitors, illustrating significant inhibition of proteolytic activities arising during the steeping period. Application of functional NTR/Trx to liquid feed reductively increased the solubility of known and potentially new Trx-target proteins, e.g., outer membrane protein X, and their susceptibility to proteolysis. Thus, the NTR/Trx system exhibits important potential as a feed additive to enhance nutrient digestibility in monogastric animals.",
keywords = "barley proteome, mass spectrometry, NADPH-dependent thioredoxin reductase/thioredoxin system, protease inhibitors",
author = "Abida Sultan and Birgit Andersen and Christensen, {Jesper Bjerg} and Poulsen, {Hanne Damgaard} and Birte Svensson and Christine Finnie",
year = "2019",
doi = "10.1021/acs.jafc.9b01708",
language = "English",
volume = "67",
pages = "6432--6444",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "22",

}

Quantitative Proteomics Analysis of Barley-Based Liquid Feed and the Effect of Protease Inhibitors and NADPH-Dependent Thioredoxin Reductase/Thioredoxin (NTR/Trx) System. / Sultan, Abida; Andersen, Birgit; Christensen, Jesper Bjerg; Poulsen, Hanne Damgaard; Svensson, Birte; Finnie, Christine.

In: Journal of Agricultural and Food Chemistry, Vol. 67, No. 22, 2019, p. 6432-6444.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Quantitative Proteomics Analysis of Barley-Based Liquid Feed and the Effect of Protease Inhibitors and NADPH-Dependent Thioredoxin Reductase/Thioredoxin (NTR/Trx) System

AU - Sultan, Abida

AU - Andersen, Birgit

AU - Christensen, Jesper Bjerg

AU - Poulsen, Hanne Damgaard

AU - Svensson, Birte

AU - Finnie, Christine

PY - 2019

Y1 - 2019

N2 - Liquid feeding strategies have been devised with the aim of enhancing grain nutrient availability for livestock. It is characterized by a steeping/soaking period that softens the grains and initiates mobilization of seed storage reserves. The present study uses 2D gel-based proteomics to investigate the role of proteolysis and reduction by thioredoxins over a 48 h steeping period by monitoring protein abundance dynamics in barley-based liquid feed samples supplemented with either protease inhibitors or NADPH-dependent thioredoxin reductase/thioredoxin (NTR/Trx). Several full-length storage proteins were only identified in the water-extractable fraction of feed containing protease inhibitors, illustrating significant inhibition of proteolytic activities arising during the steeping period. Application of functional NTR/Trx to liquid feed reductively increased the solubility of known and potentially new Trx-target proteins, e.g., outer membrane protein X, and their susceptibility to proteolysis. Thus, the NTR/Trx system exhibits important potential as a feed additive to enhance nutrient digestibility in monogastric animals.

AB - Liquid feeding strategies have been devised with the aim of enhancing grain nutrient availability for livestock. It is characterized by a steeping/soaking period that softens the grains and initiates mobilization of seed storage reserves. The present study uses 2D gel-based proteomics to investigate the role of proteolysis and reduction by thioredoxins over a 48 h steeping period by monitoring protein abundance dynamics in barley-based liquid feed samples supplemented with either protease inhibitors or NADPH-dependent thioredoxin reductase/thioredoxin (NTR/Trx). Several full-length storage proteins were only identified in the water-extractable fraction of feed containing protease inhibitors, illustrating significant inhibition of proteolytic activities arising during the steeping period. Application of functional NTR/Trx to liquid feed reductively increased the solubility of known and potentially new Trx-target proteins, e.g., outer membrane protein X, and their susceptibility to proteolysis. Thus, the NTR/Trx system exhibits important potential as a feed additive to enhance nutrient digestibility in monogastric animals.

KW - barley proteome

KW - mass spectrometry

KW - NADPH-dependent thioredoxin reductase/thioredoxin system

KW - protease inhibitors

U2 - 10.1021/acs.jafc.9b01708

DO - 10.1021/acs.jafc.9b01708

M3 - Journal article

VL - 67

SP - 6432

EP - 6444

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

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ER -