TY - JOUR
T1 - QUANTITATIVE ION-PAIR EXTRACTION OF 4(5)-METHYLIMIDAZOLE FROM CARAMEL COLOR AND ITS DETERMINATION BY REVERSED-PHASE ION-PAIR LIQUID-CHROMATOGRAPHY
AU - Thomsen, Mohens
AU - Willumsen, Dorthe
PY - 1981
Y1 - 1981
N2 - A procedure for quantitative ion-pair extraction of 4(5)-methylimidazole from caramel colour using bis(2-ethylhexyl)phosphoric acid as ion-pairing agent has been developed. Furthermore, a reversed-phase ion-pair liquid chromatographic separation method has been established to analyse the content of 4(5)-methylimidazole in the extracts. A rapid and adequate separation was achieved on a column of Nucleosil 5 C8 eluted with methanol—0.2 M potassium dihydrogen phosphate—water (32.5:25:42.5) containing 0.005 M sodium dodecanesulphonate.
This method of determination is superior in speed and repeatability to, and at higher contents of 4(5)-methylimidazole gives a better accuracy than, the World Health Organization method currently official in Denmark. The limit of detection is estimated at 4 μg/g.
AB - A procedure for quantitative ion-pair extraction of 4(5)-methylimidazole from caramel colour using bis(2-ethylhexyl)phosphoric acid as ion-pairing agent has been developed. Furthermore, a reversed-phase ion-pair liquid chromatographic separation method has been established to analyse the content of 4(5)-methylimidazole in the extracts. A rapid and adequate separation was achieved on a column of Nucleosil 5 C8 eluted with methanol—0.2 M potassium dihydrogen phosphate—water (32.5:25:42.5) containing 0.005 M sodium dodecanesulphonate.
This method of determination is superior in speed and repeatability to, and at higher contents of 4(5)-methylimidazole gives a better accuracy than, the World Health Organization method currently official in Denmark. The limit of detection is estimated at 4 μg/g.
U2 - 10.1016/S0021-9673(00)88036-X
DO - 10.1016/S0021-9673(00)88036-X
M3 - Journal article
SN - 0021-9673
VL - 211
SP - 213
EP - 221
JO - Journal of Chromatography
JF - Journal of Chromatography
IS - 2
ER -