Quantitative detection of DNA by autocatalytic enlargement of hybridized gold nanoprobes

Zongrui Zhan, Cuong Cao, Sang Jun Sim

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Quantitative detection of specific viral DNA has become a pressing issue for the earlier clinical diagnosis of viral infectious diseases. Therefore, in this paper, we report a simple, sensitive, and inexpensive quantitative approach for DNA detection based on the autocatalytic Au deposition of gold nanoprobes via the surface reduction of AuCl4− to Au0 on their surface in the presence of ascorbic acid (AA) and cetyltrimethylammonium bromide (CTAB). On this basis, signal enhancements in the absorbance intensity and kinetic behavior of gold enlargement in the aqueous phase have been well investigated and explained for the selection of analytical parameters. To achieve high sensitivity, magnetic particles conjugated with capture probes (PMPs) were employed for the collection of gold nanoprobes. After denaturated by ion a pH 11 solution, the amplified signals of gold nanoprobes, which is proportional to the concentration of the target DNA, could easily be confirmed by a UV–vis scanning spectrophotometer. Limit of detection could be obtained as low as 1.0 fM by this simple method.
    Original languageEnglish
    JournalBiosensors and Bioelectronics
    Volume26
    Issue number2
    Pages (from-to)511-516
    ISSN0956-5663
    DOIs
    Publication statusE-pub ahead of print - 2010

    Keywords

    • Gold nanoprobe
    • DNA quantitative detection
    • Autocatalytic enlargement
    • Ascorbic acid
    • Aqueous phase

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