TY - JOUR
T1 - Quantitative analysis of flavonols, flavones, and flavanones in fruits, vegetables and beverages by high-performance liquid chromatography with photo-diode array and mass spectrometric detection
AU - Justesen, U.
AU - Knuthsen, Pia
AU - Leth, Torben
PY - 1998
Y1 - 1998
N2 - A high-performance liquid chromatographic (HPLC) separation method viith photo-diode array (PDA) and mass spectrometric (MS) detection was developed to determine and quantify flavonols, flavones, and flavanones in fruits, vegetables and beverages. The compounds were analysed as aglycones, obtained after acid hydrolysis of freeze-dried food material. Identification was based on retention time, UV and mass spectra by comparison with commercial standards, and the UV peak areas were used for quantitation of the flavonoid contents. Examples of HPLC-MS analyses of orange pulp, tomato, and apple are presented. The method has been used to screen foods on the Danish market, and the contents of flavones, flavonols, and flavanones were measured. (C) 1998 Elsevier Science B.V.
AB - A high-performance liquid chromatographic (HPLC) separation method viith photo-diode array (PDA) and mass spectrometric (MS) detection was developed to determine and quantify flavonols, flavones, and flavanones in fruits, vegetables and beverages. The compounds were analysed as aglycones, obtained after acid hydrolysis of freeze-dried food material. Identification was based on retention time, UV and mass spectra by comparison with commercial standards, and the UV peak areas were used for quantitation of the flavonoid contents. Examples of HPLC-MS analyses of orange pulp, tomato, and apple are presented. The method has been used to screen foods on the Danish market, and the contents of flavones, flavonols, and flavanones were measured. (C) 1998 Elsevier Science B.V.
M3 - Journal article
SN - 0021-9673
VL - 799
SP - 101
EP - 110
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -