Quantifying Force and Viscoelasticity Inside Living Cells Using an Active–Passive Calibrated Optical Trap

Research output: Chapter in Book/Report/Conference proceedingBook chapter – Annual report year: 2017Researchpeer-review

Standard

Quantifying Force and Viscoelasticity Inside Living Cells Using an Active–Passive Calibrated Optical Trap. / Ritter, Christine M. ; Maes, Josep ; Oddershede, Lene; Berg-Sørensen, Kirstine.

Optical Tweezers: Methods and Protocols. ed. / Arne Gennerich. 2017. p. 513-536 (Methods in Molecular Biology, Vol. 1486).

Research output: Chapter in Book/Report/Conference proceedingBook chapter – Annual report year: 2017Researchpeer-review

Harvard

Ritter, CM, Maes, J, Oddershede, L & Berg-Sørensen, K 2017, Quantifying Force and Viscoelasticity Inside Living Cells Using an Active–Passive Calibrated Optical Trap. in A Gennerich (ed.), Optical Tweezers: Methods and Protocols. Methods in Molecular Biology, vol. 1486, pp. 513-536. https://doi.org/10.1007/978-1-4939-6421-5_20

APA

Ritter, C. M., Maes, J., Oddershede, L., & Berg-Sørensen, K. (2017). Quantifying Force and Viscoelasticity Inside Living Cells Using an Active–Passive Calibrated Optical Trap. In A. Gennerich (Ed.), Optical Tweezers: Methods and Protocols (pp. 513-536). Methods in Molecular Biology, Vol.. 1486 https://doi.org/10.1007/978-1-4939-6421-5_20

CBE

Ritter CM, Maes J, Oddershede L, Berg-Sørensen K. 2017. Quantifying Force and Viscoelasticity Inside Living Cells Using an Active–Passive Calibrated Optical Trap. Gennerich A, editor. In Optical Tweezers: Methods and Protocols. pp. 513-536. (Methods in Molecular Biology, Vol. 1486). https://doi.org/10.1007/978-1-4939-6421-5_20

MLA

Ritter, Christine M. et al. "Quantifying Force and Viscoelasticity Inside Living Cells Using an Active–Passive Calibrated Optical Trap". Gennerich, Arne (ed.). Optical Tweezers: Methods and Protocols. Chapter 20, (Methods in Molecular Biology, Vol. 1486). 2017, 513-536. https://doi.org/10.1007/978-1-4939-6421-5_20

Vancouver

Ritter CM, Maes J, Oddershede L, Berg-Sørensen K. Quantifying Force and Viscoelasticity Inside Living Cells Using an Active–Passive Calibrated Optical Trap. In Gennerich A, editor, Optical Tweezers: Methods and Protocols. 2017. p. 513-536. (Methods in Molecular Biology, Vol. 1486). https://doi.org/10.1007/978-1-4939-6421-5_20

Author

Ritter, Christine M. ; Maes, Josep ; Oddershede, Lene ; Berg-Sørensen, Kirstine. / Quantifying Force and Viscoelasticity Inside Living Cells Using an Active–Passive Calibrated Optical Trap. Optical Tweezers: Methods and Protocols. editor / Arne Gennerich. 2017. pp. 513-536 (Methods in Molecular Biology, Vol. 1486).

Bibtex

@inbook{a1563768d65241a897457462b2153d50,
title = "Quantifying Force and Viscoelasticity Inside Living Cells Using an Active–Passive Calibrated Optical Trap",
abstract = "As described in the previous chapters, optical tweezers have become a tool of precision for in vitro single-molecule investigations, where the single molecule of interest most often is studied in purified form in an experimental assay with a well-controlled fluidic environment. A well-controlled fluidic environment implies that the physical properties of the liquid, most notably the viscosity, are known and the fluidic environment can, for calibrational purposes, be treated as a simple liquid. In vivo, however, optical tweezers have primarily been used as a tool of manipulation and not so often for precise quantitative force measurements, due to the unknown value of the spring constant of the optical trap formed within the cell’s viscoelastic cytoplasm.Here, we describe amethod for utilizing optical tweezers for quantitative in vivo force measurements. The experimental protocol and the protocol for data analysis rely on two types of experiments, passive observation of the thermal motion of a trapped object inside a living cell, followed by observations of the response of the trapped object when subject to controlled oscillations of the optical trap. One advantage of this calibration method is that the size and refractive properties of the trapped object and the viscoelastic properties of its environment need not be known. We explain the protocol and demonstrate its use with experiments of trapped granules inside live S.pombe cells.",
keywords = "Optical Tweezers, Viscoelasticity, Cytoplasm, In Vivo, Force measurements, Spring constant",
author = "Ritter, {Christine M.} and Josep Maes and Lene Oddershede and Kirstine Berg-S{\o}rensen",
year = "2017",
doi = "10.1007/978-1-4939-6421-5_20",
language = "English",
isbn = "978-1493964192",
pages = "513--536",
editor = "Arne Gennerich",
booktitle = "Optical Tweezers: Methods and Protocols",

}

RIS

TY - CHAP

T1 - Quantifying Force and Viscoelasticity Inside Living Cells Using an Active–Passive Calibrated Optical Trap

AU - Ritter, Christine M.

AU - Maes, Josep

AU - Oddershede, Lene

AU - Berg-Sørensen, Kirstine

PY - 2017

Y1 - 2017

N2 - As described in the previous chapters, optical tweezers have become a tool of precision for in vitro single-molecule investigations, where the single molecule of interest most often is studied in purified form in an experimental assay with a well-controlled fluidic environment. A well-controlled fluidic environment implies that the physical properties of the liquid, most notably the viscosity, are known and the fluidic environment can, for calibrational purposes, be treated as a simple liquid. In vivo, however, optical tweezers have primarily been used as a tool of manipulation and not so often for precise quantitative force measurements, due to the unknown value of the spring constant of the optical trap formed within the cell’s viscoelastic cytoplasm.Here, we describe amethod for utilizing optical tweezers for quantitative in vivo force measurements. The experimental protocol and the protocol for data analysis rely on two types of experiments, passive observation of the thermal motion of a trapped object inside a living cell, followed by observations of the response of the trapped object when subject to controlled oscillations of the optical trap. One advantage of this calibration method is that the size and refractive properties of the trapped object and the viscoelastic properties of its environment need not be known. We explain the protocol and demonstrate its use with experiments of trapped granules inside live S.pombe cells.

AB - As described in the previous chapters, optical tweezers have become a tool of precision for in vitro single-molecule investigations, where the single molecule of interest most often is studied in purified form in an experimental assay with a well-controlled fluidic environment. A well-controlled fluidic environment implies that the physical properties of the liquid, most notably the viscosity, are known and the fluidic environment can, for calibrational purposes, be treated as a simple liquid. In vivo, however, optical tweezers have primarily been used as a tool of manipulation and not so often for precise quantitative force measurements, due to the unknown value of the spring constant of the optical trap formed within the cell’s viscoelastic cytoplasm.Here, we describe amethod for utilizing optical tweezers for quantitative in vivo force measurements. The experimental protocol and the protocol for data analysis rely on two types of experiments, passive observation of the thermal motion of a trapped object inside a living cell, followed by observations of the response of the trapped object when subject to controlled oscillations of the optical trap. One advantage of this calibration method is that the size and refractive properties of the trapped object and the viscoelastic properties of its environment need not be known. We explain the protocol and demonstrate its use with experiments of trapped granules inside live S.pombe cells.

KW - Optical Tweezers

KW - Viscoelasticity

KW - Cytoplasm

KW - In Vivo

KW - Force measurements

KW - Spring constant

U2 - 10.1007/978-1-4939-6421-5_20

DO - 10.1007/978-1-4939-6421-5_20

M3 - Book chapter

SN - 978-1493964192

SP - 513

EP - 536

BT - Optical Tweezers: Methods and Protocols

A2 - Gennerich, Arne

ER -