Quantified pH imaging with hyperpolarized 13C‐bicarbonate: Quantified pH Imaging with Hyperpolarized Bicarbonate

David Johannes Scholz, Martin A. Janich, Ulrich Köllisch, Rolf F. Schulte, Jan Henrik Ardenkjær-Larsen, Annette Frank, Axel Haase, Markus Schwaiger, Marion I. Menzel

Research output: Contribution to journalJournal articlepeer-review

Abstract

Because pH plays a crucial role in several diseases, it is desirable to measure pH in vivo noninvasively and in a spatially localized manner. Spatial maps of pH were quantified in vitro, with a focus on method‐based errors, and applied in vivo. In vitro and in vivo 13C mapping were performed for various flip angles for bicarbonate (BiC) and CO2 with spectral‐spatial excitation and spiral readout in healthy Lewis rats in five slices. Acute subcutaneous sterile inflammation was induced with Concanavalin A in the right leg of Buffalo rats. pH and proton images were measured 2 h after induction. After optimizing the signal to noise ratio of the hyperpolarized 13C‐bicarbonate, error estimation of the spectral‐spatial excited spectrum reveals that the method covers the biologically relevant pH range of 6 to 8 with low pH error (<0.2). Quantification of pH maps shows negligible impact of the residual bicarbonate signal. pH maps reflect the induction of acute metabolic alkalosis. Inflamed, infected regions exhibit lower pH. Hyperpolarized 13C‐bicarbonate pH mapping was shown to be sensitive in the biologically relevant pH range. The mapping of pH was applied to healthy in vivo organs and interpreted within inflammation and acute metabolic alkalosis models. Magn Reson Med 73:2274–2282, 2015. © 2014 Wiley Periodicals, Inc.
Original languageEnglish
JournalMagnetic Resonance in Medicine
Volume73
Issue number6
Pages (from-to)2274-2282
ISSN0740-3194
DOIs
Publication statusPublished - 2015

Keywords

  • Hyperpolarized 13C
  • NMR spectroscopy
  • pH
  • Bicarbonate
  • MRI
  • In vivo

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