Quantification of specific E. coli in gut mucosa from Crohn's disease patients

Stina Rikke Jensen; Lisbeth Nielsen Fink; Carsten Struve; Claus Sternberg; Jens Bo Andersen; Jørn Brynskov; Ole Haagen Nielsen; Susanne Brix Pedersen

doi:10.1016/j.mimet.2011.04.002

Quantification of specific E. coli in gut mucosa from Crohn's disease patients

Stina Rikke Jensen
, Lisbeth Nielsen Fink
, Carsten Struve
, Claus Sternberg
, Jens Bo Andersen
, Jørn Brynskov
, Ole Haagen Nielsen
, Susanne Brix Pedersen

National Food Institute

Statens Serum Institut
Copenhagen University Hospital Herlev and Gentofte

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.

Original language	English
Journal	Journal of Microbiological Methods
Volume	86
Issue number	1
Pages (from-to)	111-114
ISSN	0167-7012
DOIs	https://doi.org/10.1016/j.mimet.2011.04.002
Publication status	Published - 2011

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10.1016/j.mimet.2011.04.002

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title = "Quantification of specific E. coli in gut mucosa from Crohn's disease patients",

abstract = "We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.",

author = "Jensen, \{Stina Rikke\} and Fink, \{Lisbeth Nielsen\} and Carsten Struve and Claus Sternberg and Andersen, \{Jens Bo\} and J{\o}rn Brynskov and Nielsen, \{Ole Haagen\} and Pedersen, \{Susanne Brix\}",

year = "2011",

doi = "10.1016/j.mimet.2011.04.002",

language = "English",

volume = "86",

pages = "111--114",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

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TY - JOUR

T1 - Quantification of specific E. coli in gut mucosa from Crohn's disease patients

AU - Jensen, Stina Rikke

AU - Fink, Lisbeth Nielsen

AU - Struve, Carsten

AU - Sternberg, Claus

AU - Andersen, Jens Bo

AU - Brynskov, Jørn

AU - Nielsen, Ole Haagen

AU - Pedersen, Susanne Brix

PY - 2011

Y1 - 2011

N2 - We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.

AB - We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.

U2 - 10.1016/j.mimet.2011.04.002

DO - 10.1016/j.mimet.2011.04.002

M3 - Journal article

C2 - 21504765

SN - 0167-7012

VL - 86

SP - 111

EP - 114

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 1

ER -

Quantification of specific E. coli in gut mucosa from Crohn's disease patients

Abstract

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