Quantification of specific E. coli in gut mucosa from Crohn's disease patients

Stina Rikke Jensen, Lisbeth Nielsen Fink, Carsten Struve, Claus Sternberg, Jens Bo Andersen, Jørn Brynskov, Ole Haagen Nielsen, Susanne Brix Pedersen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.
Original languageEnglish
JournalJournal of Microbiological Methods
Volume86
Issue number1
Pages (from-to)111-114
ISSN0167-7012
DOIs
Publication statusPublished - 2011

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