Quantification of folate in food using deconjugase of plant origin combined with LC-MS/MS

A method comparison of a large and diverse sample set

Petra Ložnjak*, Lisa Striegel, Rocío I. Díaz De la Garza, Michael Rychlik, Jette Jakobsen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A round robin comparison was performed in order to test the performance of a recently developed LC-MS/MS method for quantification of 6 folate forms. Eighty-nine samples representing the food groups of fruits, vegetables, legumes, cereals, dairy products, meat, and offal were analyzed by two LC-MS/MS methods and a microbiological assay (MA). A plant-origin deconjugase enzyme (Arabidopsis thaliana) for deconjugation of folates (PE-LC-MS/MS), or animal-origin deconjugase (rat serum and chicken pancreas) (AE-LC-MS/MS) was used in the LC-MS/MS methods, each in a single enzymatic step. In contrast, the MA involved tri-enzyme extraction including human plasma as a deconjugase. A significant bias of 17% lower and 25% higher results was found when PE-LC-MS/MS was compared to MA and AE-LC-MS/MS, respectively. The PE-LC-MS/MS provides fast quantification of various folate vitamers and total folate content, which could be a proper substitute to the currently standardized but imprecise and time-consuming microbiological assay in the future.

Original languageEnglish
Article number125450
JournalFood Chemistry
Volume305
Number of pages10
ISSN0308-8146
DOIs
Publication statusPublished - 2020

Keywords

  • 10-Formylfolic acid (PubChem CID: 3080544)
  • 5,10-Methenyltetrahydrofolate (PubChem CID: 439237)
  • 5-Formyltetrahydrofolate (PubChem CID: 149436)
  • 5-Methyltetrahydrofolate (PubChem CID: 439234)
  • Deconjugase enzymes
  • Folates
  • Folic acid (PubChem CID: 6037)
  • Food
  • LC-MS/MS
  • Method comparison
  • Microbiological assay
  • Tetrahydrofolate (PubChem CID: 1129)

Cite this

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title = "Quantification of folate in food using deconjugase of plant origin combined with LC-MS/MS: A method comparison of a large and diverse sample set",
abstract = "A round robin comparison was performed in order to test the performance of a recently developed LC-MS/MS method for quantification of 6 folate forms. Eighty-nine samples representing the food groups of fruits, vegetables, legumes, cereals, dairy products, meat, and offal were analyzed by two LC-MS/MS methods and a microbiological assay (MA). A plant-origin deconjugase enzyme (Arabidopsis thaliana) for deconjugation of folates (PE-LC-MS/MS), or animal-origin deconjugase (rat serum and chicken pancreas) (AE-LC-MS/MS) was used in the LC-MS/MS methods, each in a single enzymatic step. In contrast, the MA involved tri-enzyme extraction including human plasma as a deconjugase. A significant bias of 17{\%} lower and 25{\%} higher results was found when PE-LC-MS/MS was compared to MA and AE-LC-MS/MS, respectively. The PE-LC-MS/MS provides fast quantification of various folate vitamers and total folate content, which could be a proper substitute to the currently standardized but imprecise and time-consuming microbiological assay in the future.",
keywords = "10-Formylfolic acid (PubChem CID: 3080544), 5,10-Methenyltetrahydrofolate (PubChem CID: 439237), 5-Formyltetrahydrofolate (PubChem CID: 149436), 5-Methyltetrahydrofolate (PubChem CID: 439234), Deconjugase enzymes, Folates, Folic acid (PubChem CID: 6037), Food, LC-MS/MS, Method comparison, Microbiological assay, Tetrahydrofolate (PubChem CID: 1129)",
author = "Petra Ložnjak and Lisa Striegel and {D{\'i}az De la Garza}, {Roc{\'i}o I.} and Michael Rychlik and Jette Jakobsen",
year = "2020",
doi = "10.1016/j.foodchem.2019.125450",
language = "English",
volume = "305",
journal = "Food Chemistry",
issn = "0308-8146",
publisher = "Elsevier",

}

Quantification of folate in food using deconjugase of plant origin combined with LC-MS/MS : A method comparison of a large and diverse sample set. / Ložnjak, Petra; Striegel, Lisa; Díaz De la Garza, Rocío I.; Rychlik, Michael; Jakobsen, Jette.

In: Food Chemistry, Vol. 305, 125450, 2020.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Quantification of folate in food using deconjugase of plant origin combined with LC-MS/MS

T2 - A method comparison of a large and diverse sample set

AU - Ložnjak, Petra

AU - Striegel, Lisa

AU - Díaz De la Garza, Rocío I.

AU - Rychlik, Michael

AU - Jakobsen, Jette

PY - 2020

Y1 - 2020

N2 - A round robin comparison was performed in order to test the performance of a recently developed LC-MS/MS method for quantification of 6 folate forms. Eighty-nine samples representing the food groups of fruits, vegetables, legumes, cereals, dairy products, meat, and offal were analyzed by two LC-MS/MS methods and a microbiological assay (MA). A plant-origin deconjugase enzyme (Arabidopsis thaliana) for deconjugation of folates (PE-LC-MS/MS), or animal-origin deconjugase (rat serum and chicken pancreas) (AE-LC-MS/MS) was used in the LC-MS/MS methods, each in a single enzymatic step. In contrast, the MA involved tri-enzyme extraction including human plasma as a deconjugase. A significant bias of 17% lower and 25% higher results was found when PE-LC-MS/MS was compared to MA and AE-LC-MS/MS, respectively. The PE-LC-MS/MS provides fast quantification of various folate vitamers and total folate content, which could be a proper substitute to the currently standardized but imprecise and time-consuming microbiological assay in the future.

AB - A round robin comparison was performed in order to test the performance of a recently developed LC-MS/MS method for quantification of 6 folate forms. Eighty-nine samples representing the food groups of fruits, vegetables, legumes, cereals, dairy products, meat, and offal were analyzed by two LC-MS/MS methods and a microbiological assay (MA). A plant-origin deconjugase enzyme (Arabidopsis thaliana) for deconjugation of folates (PE-LC-MS/MS), or animal-origin deconjugase (rat serum and chicken pancreas) (AE-LC-MS/MS) was used in the LC-MS/MS methods, each in a single enzymatic step. In contrast, the MA involved tri-enzyme extraction including human plasma as a deconjugase. A significant bias of 17% lower and 25% higher results was found when PE-LC-MS/MS was compared to MA and AE-LC-MS/MS, respectively. The PE-LC-MS/MS provides fast quantification of various folate vitamers and total folate content, which could be a proper substitute to the currently standardized but imprecise and time-consuming microbiological assay in the future.

KW - 10-Formylfolic acid (PubChem CID: 3080544)

KW - 5,10-Methenyltetrahydrofolate (PubChem CID: 439237)

KW - 5-Formyltetrahydrofolate (PubChem CID: 149436)

KW - 5-Methyltetrahydrofolate (PubChem CID: 439234)

KW - Deconjugase enzymes

KW - Folates

KW - Folic acid (PubChem CID: 6037)

KW - Food

KW - LC-MS/MS

KW - Method comparison

KW - Microbiological assay

KW - Tetrahydrofolate (PubChem CID: 1129)

U2 - 10.1016/j.foodchem.2019.125450

DO - 10.1016/j.foodchem.2019.125450

M3 - Journal article

VL - 305

JO - Food Chemistry

JF - Food Chemistry

SN - 0308-8146

M1 - 125450

ER -