Quantification of Brain Access of Exendin-4 in the C57BL Mouse Model by SPIM Fluorescence Imaging and the Allen Mouse Brain Reference Model

Casper Bo Jensen, Anna Secher, Jacob Hecksher-Sørensen, Knut Conradsen, Rasmus Larsen

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review


With the recent advance in 3D microscopy such as Single Plane Illumination Microscopy (SPIM) it is possible to obtain high resolution image volumes of the entire mouse brain. These data can be used to study the access of several peptides such as the glucagon-like peptide-1 (GLP-1) analogue Exendin-4, into the brain with the aim of developing medication for obesity. To investigate mode of action of the medication it is important to identify the specific anatomical brain nuclei that are targeted by the compound. Such segmentations can be obtained using an annotated digital brain atlas. We construct a SPIM brain atlas based on the Allen mouse brain 3D reference model and use it to analyze the access of peripherally injected Exendin-4 into the brain compared to a negative control group. The constructed atlas consists of an average SPIM volume obtained from eight C57BL mouse brains using group-wise registration. A cross-modality registration is performed between the constructed average volume and the Allen mouse brain reference model to allow propagation of annotations to the SPIM average brain. Finally, manual corrections of the annotations are performed and validated by visual inspection. The study shows that Exendin-4 have access to brain regions such as the arcuate hypothalamic nucleus and the nucleus of the solitary tract, which are areas involved in regulating food intake.
Original languageEnglish
Title of host publicationImage Analysis : 19th Scandinavian Conference, SCIA 2015 Copenhagen, Denmark, June 15–17, 2015 Proceedings
Number of pages10
PublisherSpringer Science+Business Media
Publication date2015
ISBN (Print)978-3-319-19664-0
ISBN (Electronic)978-3-319-19665-7
Publication statusPublished - 2015
Event19th Scandinavian Conference on Image Analysis - Copenhagen, Denmark
Duration: 15 Jun 201517 Jun 2015
Conference number: 19


Conference19th Scandinavian Conference on Image Analysis
Internet address
SeriesLecture Notes in Computer Science


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