Quantification and Evaluation of Infectivity of Shiga Toxin-Encoding Bacteriophages in Beef and Salad

Lejla Imamovic; Maite Muniesa

doi:10.1128/AEM.02703-10

Quantification and Evaluation of Infectivity of Shiga Toxin-Encoding Bacteriophages in Beef and Salad

Lejla Imamovic, Maite Muniesa

University of Barcelona

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Abstract

Stx bacteriophages in 68 samples of beef and salad were quantified by real-time quantitative PCR (qPCR). Stx phages from the samples were propagated in Escherichia coli C600, E. coli O157:H7, and Shigella strains and further quantified. Fifty percent of the samples carried infectious Stx phages that were isolated from plaques generated by lysis.

Original language	English
Journal	Applied and Environmental Microbiology
Volume	77
Issue number	10
Pages (from-to)	3536-3540
ISSN	0099-2240
DOIs	https://doi.org/10.1128/AEM.02703-10
Publication status	Published - 2011
Externally published	Yes

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DO - 10.1128/AEM.02703-10

M3 - Journal article

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Quantification and Evaluation of Infectivity of Shiga Toxin-Encoding Bacteriophages in Beef and Salad

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