Pyrrolizidine alkaloids in honey: Quantification with and without standards

Tingting Wang, Henrik Lauritz Frandsen, Natasha Roed Christiansson, Sofie Elisabeth Rosendal, Mikael Pedersen, Jørn Smedsgaard*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Quantification of a large number of compounds in the absence of authentic standards is always a challenge. More than 600 pyrrolizidine alkaloids (PAs) have been found in plants however only limited number of PAs standards are commercially available. As PAs are the most widely distributed natural plant toxins with threat to human health, risk assessment calls for quantitative analytical methods with a wide scope including PAs without available standards. In this study, a method was developed that allows simultaneous quantification of 12 PAs in the honey samples by using HPLC-HRMS with authentic standards. This method was further extended to screen for other potential PAs in the honey using multi-target screening combined with a quantitative prediction model in the cases that authentic PAs were not available. The prediction model was subsequently validated by cross-validation and additional PAs standards which were not included in the model. The maximum concentration prediction error was 50.8%.
Original languageEnglish
JournalFood Control
Volume98
Pages (from-to)227-237
ISSN0956-7135
DOIs
Publication statusPublished - 2019

Keywords

  • Pyrrolizidine alkaloids
  • Validation
  • Quantification without standards
  • Screening
  • Honey
  • HPLC-HRMS

Cite this

Wang, Tingting ; Frandsen, Henrik Lauritz ; Roed Christiansson, Natasha ; Rosendal, Sofie Elisabeth ; Pedersen, Mikael ; Smedsgaard, Jørn. / Pyrrolizidine alkaloids in honey: Quantification with and without standards. In: Food Control. 2019 ; Vol. 98. pp. 227-237.
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abstract = "Quantification of a large number of compounds in the absence of authentic standards is always a challenge. More than 600 pyrrolizidine alkaloids (PAs) have been found in plants however only limited number of PAs standards are commercially available. As PAs are the most widely distributed natural plant toxins with threat to human health, risk assessment calls for quantitative analytical methods with a wide scope including PAs without available standards. In this study, a method was developed that allows simultaneous quantification of 12 PAs in the honey samples by using HPLC-HRMS with authentic standards. This method was further extended to screen for other potential PAs in the honey using multi-target screening combined with a quantitative prediction model in the cases that authentic PAs were not available. The prediction model was subsequently validated by cross-validation and additional PAs standards which were not included in the model. The maximum concentration prediction error was 50.8{\%}.",
keywords = "Pyrrolizidine alkaloids, Validation, Quantification without standards, Screening, Honey, HPLC-HRMS",
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year = "2019",
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Pyrrolizidine alkaloids in honey: Quantification with and without standards. / Wang, Tingting; Frandsen, Henrik Lauritz; Roed Christiansson, Natasha ; Rosendal, Sofie Elisabeth; Pedersen, Mikael; Smedsgaard, Jørn.

In: Food Control, Vol. 98, 2019, p. 227-237.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Pyrrolizidine alkaloids in honey: Quantification with and without standards

AU - Wang, Tingting

AU - Frandsen, Henrik Lauritz

AU - Roed Christiansson, Natasha

AU - Rosendal, Sofie Elisabeth

AU - Pedersen, Mikael

AU - Smedsgaard, Jørn

PY - 2019

Y1 - 2019

N2 - Quantification of a large number of compounds in the absence of authentic standards is always a challenge. More than 600 pyrrolizidine alkaloids (PAs) have been found in plants however only limited number of PAs standards are commercially available. As PAs are the most widely distributed natural plant toxins with threat to human health, risk assessment calls for quantitative analytical methods with a wide scope including PAs without available standards. In this study, a method was developed that allows simultaneous quantification of 12 PAs in the honey samples by using HPLC-HRMS with authentic standards. This method was further extended to screen for other potential PAs in the honey using multi-target screening combined with a quantitative prediction model in the cases that authentic PAs were not available. The prediction model was subsequently validated by cross-validation and additional PAs standards which were not included in the model. The maximum concentration prediction error was 50.8%.

AB - Quantification of a large number of compounds in the absence of authentic standards is always a challenge. More than 600 pyrrolizidine alkaloids (PAs) have been found in plants however only limited number of PAs standards are commercially available. As PAs are the most widely distributed natural plant toxins with threat to human health, risk assessment calls for quantitative analytical methods with a wide scope including PAs without available standards. In this study, a method was developed that allows simultaneous quantification of 12 PAs in the honey samples by using HPLC-HRMS with authentic standards. This method was further extended to screen for other potential PAs in the honey using multi-target screening combined with a quantitative prediction model in the cases that authentic PAs were not available. The prediction model was subsequently validated by cross-validation and additional PAs standards which were not included in the model. The maximum concentration prediction error was 50.8%.

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KW - Validation

KW - Quantification without standards

KW - Screening

KW - Honey

KW - HPLC-HRMS

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DO - 10.1016/j.foodcont.2018.11.033

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SP - 227

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SN - 0956-7135

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