Pyrolytic carbon microelectrodes for impedance based cell sensing

Yasmin Mohamed Hassan; Claudia Caviglia; Suhith Hemanth; David Mackenzie; Dirch Hjorth Petersen; Stephan Sylvest Keller

doi:10.1149/07201.0035ecst

Pyrolytic carbon microelectrodes for impedance based cell sensing

Yasmin Mohamed Hassan, Claudia Caviglia, Suhith Hemanth, David Mackenzie, Dirch Hjorth Petersen, Stephan Sylvest Keller

Research output: Contribution to journal › Conference article › Research › peer-review

Abstract

Electrically conductive glass-like carbon structures can be obtained from a polymer template through a pyrolysis process. These structures can be used as electrodes for bio sensing applications such as electrochemical evaluation of cell adhesion and proliferation. This study focuses on the optimization of two dimensional (2D) pyrolytic carbon microelectrodes with the carbon MEMS (C-MEMS) process using the negative epoxy photoresist SU-8. Different electrochemical microchips with carbon working (WE) and counter electrode (CE) were fabricated. More specifically, pyrolysis process was optimized to decrease the resistivity of the resulting carbon material and improve the performance in cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Finally, EIS was used to monitor adhesion and proliferation of HeLa cells.

Original language	English
Journal	E C S Transactions
Volume	72
Issue number	1
Pages (from-to)	35-44
ISSN	1938-5862
DOIs	https://doi.org/10.1149/07201.0035ecst
Publication status	Published - 2016
Event	The 229th ECS Meeting - San Diego, United States Duration: 29 May 2016 → 2 Jun 2016 Conference number: 229 http://www.electrochem.org/229

Conference

Conference	The 229th ECS Meeting
Number	229
Country/Territory	United States
City	San Diego
Period	29/05/2016 → 02/06/2016
Internet address	http://www.electrochem.org/229

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title = "Pyrolytic carbon microelectrodes for impedance based cell sensing",

abstract = "Electrically conductive glass-like carbon structures can be obtained from a polymer template through a pyrolysis process. These structures can be used as electrodes for bio sensing applications such as electrochemical evaluation of cell adhesion and proliferation. This study focuses on the optimization of two dimensional (2D) pyrolytic carbon microelectrodes with the carbon MEMS (C-MEMS) process using the negative epoxy photoresist SU-8. Different electrochemical microchips with carbon working (WE) and counter electrode (CE) were fabricated. More specifically, pyrolysis process was optimized to decrease the resistivity of the resulting carbon material and improve the performance in cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Finally, EIS was used to monitor adhesion and proliferation of HeLa cells. ",

author = "Hassan, {Yasmin Mohamed} and Claudia Caviglia and Suhith Hemanth and David Mackenzie and Petersen, {Dirch Hjorth} and Keller, {Stephan Sylvest}",

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doi = "10.1149/07201.0035ecst",

language = "English",

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AU - Hassan, Yasmin Mohamed

AU - Caviglia, Claudia

AU - Hemanth, Suhith

AU - Mackenzie, David

AU - Petersen, Dirch Hjorth

AU - Keller, Stephan Sylvest

N1 - Conference code: 229

PY - 2016

Y1 - 2016

N2 - Electrically conductive glass-like carbon structures can be obtained from a polymer template through a pyrolysis process. These structures can be used as electrodes for bio sensing applications such as electrochemical evaluation of cell adhesion and proliferation. This study focuses on the optimization of two dimensional (2D) pyrolytic carbon microelectrodes with the carbon MEMS (C-MEMS) process using the negative epoxy photoresist SU-8. Different electrochemical microchips with carbon working (WE) and counter electrode (CE) were fabricated. More specifically, pyrolysis process was optimized to decrease the resistivity of the resulting carbon material and improve the performance in cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Finally, EIS was used to monitor adhesion and proliferation of HeLa cells.

AB - Electrically conductive glass-like carbon structures can be obtained from a polymer template through a pyrolysis process. These structures can be used as electrodes for bio sensing applications such as electrochemical evaluation of cell adhesion and proliferation. This study focuses on the optimization of two dimensional (2D) pyrolytic carbon microelectrodes with the carbon MEMS (C-MEMS) process using the negative epoxy photoresist SU-8. Different electrochemical microchips with carbon working (WE) and counter electrode (CE) were fabricated. More specifically, pyrolysis process was optimized to decrease the resistivity of the resulting carbon material and improve the performance in cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Finally, EIS was used to monitor adhesion and proliferation of HeLa cells.

U2 - 10.1149/07201.0035ecst

DO - 10.1149/07201.0035ecst

M3 - Conference article

SN - 1938-5862

VL - 72

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JO - E C S Transactions

JF - E C S Transactions

IS - 1

T2 - The 229th ECS Meeting

Y2 - 29 May 2016 through 2 June 2016

ER -

Pyrolytic carbon microelectrodes for impedance based cell sensing

Abstract

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