Abstract
By a suppression subtractive hybridization based method, nine novel Mycobacterium avium subsp. para tuberculosis (M. para tuberculosis) fragments of between 318 and 596 bp have been identified and characterized. Database search revealed little or no similarity with other mycobacteria. The uniqueness and diagnostic potential of seven of these fragments in relation to M. paratuberculosis closest relative Mycobacterium avium subsp. avium (M. avium) was confirmed by species-specific PCR and Southern blot. Furthermore, RT-PCR indicated that eight of the nine fragments originate from areas of the genome that are expressed in vitro.
| Original language | English |
|---|---|
| Journal | FEMS Microbiology Letters |
| Volume | 214 |
| Issue number | 2 |
| Pages (from-to) | 199-203 |
| ISSN | 0378-1097 |
| DOIs | |
| Publication status | Published - 2002 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- Mycobacterium paratuberculosis
- subtractive hybridization
- RT-PCR
- species-specific elements
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