Putative in vitro expressed gene fragments unique to Mycobacterium avium subspecies para tuberculosis

Kirstine Klitgaard Nielsen, Peter Ahrens

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    By a suppression subtractive hybridization based method, nine novel Mycobacterium avium subsp. para tuberculosis (M. para tuberculosis) fragments of between 318 and 596 bp have been identified and characterized. Database search revealed little or no similarity with other mycobacteria. The uniqueness and diagnostic potential of seven of these fragments in relation to M. paratuberculosis closest relative Mycobacterium avium subsp. avium (M. avium) was confirmed by species-specific PCR and Southern blot. Furthermore, RT-PCR indicated that eight of the nine fragments originate from areas of the genome that are expressed in vitro.
    Original languageEnglish
    JournalFEMS Microbiology Letters
    Volume214
    Issue number2
    Pages (from-to)199-203
    ISSN0378-1097
    DOIs
    Publication statusPublished - 2002

    Keywords

    • Mycobacterium paratuberculosis
    • subtractive hybridization
    • RT-PCR
    • species-specific elements

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