Putative in vitro expressed gene fragments unique to Mycobacterium avium subspecies para tuberculosis

Kirstine Klitgaard Nielsen; Peter Ahrens

doi:10.1016/S0378-1097(02)00859-5

Putative in vitro expressed gene fragments unique to Mycobacterium avium subspecies para tuberculosis

Kirstine Klitgaard Nielsen, Peter Ahrens

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

By a suppression subtractive hybridization based method, nine novel Mycobacterium avium subsp. para tuberculosis (M. para tuberculosis) fragments of between 318 and 596 bp have been identified and characterized. Database search revealed little or no similarity with other mycobacteria. The uniqueness and diagnostic potential of seven of these fragments in relation to M. paratuberculosis closest relative Mycobacterium avium subsp. avium (M. avium) was confirmed by species-specific PCR and Southern blot. Furthermore, RT-PCR indicated that eight of the nine fragments originate from areas of the genome that are expressed in vitro.

Original language	English
Journal	FEMS Microbiology Letters
Volume	214
Issue number	2
Pages (from-to)	199-203
ISSN	0378-1097
DOIs	https://doi.org/10.1016/S0378-1097(02)00859-5
Publication status	Published - 2002

Keywords

Mycobacterium paratuberculosis
subtractive hybridization
RT-PCR
species-specific elements

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0378-1097(02)00859-5

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abstract = "By a suppression subtractive hybridization based method, nine novel Mycobacterium avium subsp. para tuberculosis (M. para tuberculosis) fragments of between 318 and 596 bp have been identified and characterized. Database search revealed little or no similarity with other mycobacteria. The uniqueness and diagnostic potential of seven of these fragments in relation to M. paratuberculosis closest relative Mycobacterium avium subsp. avium (M. avium) was confirmed by species-specific PCR and Southern blot. Furthermore, RT-PCR indicated that eight of the nine fragments originate from areas of the genome that are expressed in vitro.",

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AB - By a suppression subtractive hybridization based method, nine novel Mycobacterium avium subsp. para tuberculosis (M. para tuberculosis) fragments of between 318 and 596 bp have been identified and characterized. Database search revealed little or no similarity with other mycobacteria. The uniqueness and diagnostic potential of seven of these fragments in relation to M. paratuberculosis closest relative Mycobacterium avium subsp. avium (M. avium) was confirmed by species-specific PCR and Southern blot. Furthermore, RT-PCR indicated that eight of the nine fragments originate from areas of the genome that are expressed in vitro.

KW - Mycobacterium paratuberculosis

KW - subtractive hybridization

KW - RT-PCR

KW - species-specific elements

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JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

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ER -

Putative in vitro expressed gene fragments unique to Mycobacterium avium subspecies para tuberculosis

Abstract

Keywords

UN SDGs

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