TY - JOUR
T1 - Purification and characterization of xylitol dehydrogenase from Fusarium oxysporum
AU - Panagiotou, Gianni
AU - Kekos, D.
AU - Macris, B.J.
AU - Christakopoulos, Paul
PY - 2002
Y1 - 2002
N2 - An NAD(+)-dependent xylitol dehydrogenase (XDH) from Fusarium oxysporum, a key enzyme in the conversion of xylose to ethanol, was purified to homogeneity and characterised. It was homodimeric with a subunit of M-r 48 000, and pI 3.6. It was optimally active at 45degreesC and pH 9-10. It was fully stable at pH 6-7 for 24 h and 30degreesC. K-m values for D-xylitol and NAD(+) were 94 mM and 0.14 mM, respectively. Mn2+ at 10 mM increased XDH activity 2-fold and Cu2+ at 10 mM inhibited activity completely.
AB - An NAD(+)-dependent xylitol dehydrogenase (XDH) from Fusarium oxysporum, a key enzyme in the conversion of xylose to ethanol, was purified to homogeneity and characterised. It was homodimeric with a subunit of M-r 48 000, and pI 3.6. It was optimally active at 45degreesC and pH 9-10. It was fully stable at pH 6-7 for 24 h and 30degreesC. K-m values for D-xylitol and NAD(+) were 94 mM and 0.14 mM, respectively. Mn2+ at 10 mM increased XDH activity 2-fold and Cu2+ at 10 mM inhibited activity completely.
U2 - 10.1023/A:1021317614948
DO - 10.1023/A:1021317614948
M3 - Journal article
VL - 24
SP - 2089
EP - 2092
JO - Biotechnology Letters
JF - Biotechnology Letters
SN - 0141-5492
IS - 24
ER -