Pulsed laser deposition of lysozyme: the dependence on shot numbers and the angular distribution

C. Constantinescu, A. Matei, Jørgen Schou, Stela Canulescu, M. Dinescu

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The ejection of molecules from a pressed solid target of lysozyme induced by laser ablation in the UV-regime at a wavelength of 355 nm was investigated. The ablation studies were carried out in vacuum at a laser fluence of 2 J/cm2 for which a significant fraction of proteins remains intact. This was verified by matrix-assisted laser desorption ionization (MALDI) spectrometry of thin films deposited on silicon substrates. The deposition rate of lysozyme was found to decrease with the number of shots and was correlated with increasing thermal damage of the lysozyme. This was monitored by measurements of the optical reflectivity of dry lysozyme. The angular distribution of the mass deposition can be fitted well by Anisimov’s hydrodynamic model. The total deposited yield over the entire hemisphere from direct laser ablation of lysozyme was estimated from this model and found to be three orders of magnitude less than the ablated mass.
Original languageEnglish
JournalApplied Physics B
Volume113
Issue number3
Pages (from-to)367-371
ISSN0946-2171
DOIs
Publication statusPublished - 2013

Cite this

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title = "Pulsed laser deposition of lysozyme: the dependence on shot numbers and the angular distribution",
abstract = "The ejection of molecules from a pressed solid target of lysozyme induced by laser ablation in the UV-regime at a wavelength of 355 nm was investigated. The ablation studies were carried out in vacuum at a laser fluence of 2 J/cm2 for which a significant fraction of proteins remains intact. This was verified by matrix-assisted laser desorption ionization (MALDI) spectrometry of thin films deposited on silicon substrates. The deposition rate of lysozyme was found to decrease with the number of shots and was correlated with increasing thermal damage of the lysozyme. This was monitored by measurements of the optical reflectivity of dry lysozyme. The angular distribution of the mass deposition can be fitted well by Anisimov’s hydrodynamic model. The total deposited yield over the entire hemisphere from direct laser ablation of lysozyme was estimated from this model and found to be three orders of magnitude less than the ablated mass.",
author = "C. Constantinescu and A. Matei and J{\o}rgen Schou and Stela Canulescu and M. Dinescu",
year = "2013",
doi = "10.1007/s00340-013-5485-3",
language = "English",
volume = "113",
pages = "367--371",
journal = "Applied Physics B",
issn = "0946-2171",
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}

Pulsed laser deposition of lysozyme: the dependence on shot numbers and the angular distribution. / Constantinescu, C.; Matei, A.; Schou, Jørgen; Canulescu, Stela; Dinescu, M.

In: Applied Physics B, Vol. 113, No. 3, 2013, p. 367-371.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Pulsed laser deposition of lysozyme: the dependence on shot numbers and the angular distribution

AU - Constantinescu, C.

AU - Matei, A.

AU - Schou, Jørgen

AU - Canulescu, Stela

AU - Dinescu, M.

PY - 2013

Y1 - 2013

N2 - The ejection of molecules from a pressed solid target of lysozyme induced by laser ablation in the UV-regime at a wavelength of 355 nm was investigated. The ablation studies were carried out in vacuum at a laser fluence of 2 J/cm2 for which a significant fraction of proteins remains intact. This was verified by matrix-assisted laser desorption ionization (MALDI) spectrometry of thin films deposited on silicon substrates. The deposition rate of lysozyme was found to decrease with the number of shots and was correlated with increasing thermal damage of the lysozyme. This was monitored by measurements of the optical reflectivity of dry lysozyme. The angular distribution of the mass deposition can be fitted well by Anisimov’s hydrodynamic model. The total deposited yield over the entire hemisphere from direct laser ablation of lysozyme was estimated from this model and found to be three orders of magnitude less than the ablated mass.

AB - The ejection of molecules from a pressed solid target of lysozyme induced by laser ablation in the UV-regime at a wavelength of 355 nm was investigated. The ablation studies were carried out in vacuum at a laser fluence of 2 J/cm2 for which a significant fraction of proteins remains intact. This was verified by matrix-assisted laser desorption ionization (MALDI) spectrometry of thin films deposited on silicon substrates. The deposition rate of lysozyme was found to decrease with the number of shots and was correlated with increasing thermal damage of the lysozyme. This was monitored by measurements of the optical reflectivity of dry lysozyme. The angular distribution of the mass deposition can be fitted well by Anisimov’s hydrodynamic model. The total deposited yield over the entire hemisphere from direct laser ablation of lysozyme was estimated from this model and found to be three orders of magnitude less than the ablated mass.

U2 - 10.1007/s00340-013-5485-3

DO - 10.1007/s00340-013-5485-3

M3 - Journal article

VL - 113

SP - 367

EP - 371

JO - Applied Physics B

JF - Applied Physics B

SN - 0946-2171

IS - 3

ER -