Pulmonary toxicity of two different multi-walled carbon nanotubes in rat: Comparison between intratracheal instillation and inhalation exposure

Research output: Contribution to journalJournal article – Annual report year: 2019Researchpeer-review

  • Author: Gaté, Laurent

    Institut National de Recherche et de Securité, France

  • Author: Knudsen, Kristina Bram

    National Research Centre for the Working Environment, Denmark

  • Author: Seidel, Carole

    Institut National de Recherche et de Securité, France

  • Author: Berthing, Trine

    National Research Centre for the Working Environment, Denmark

  • Author: Chézeau, Laëtitia

    Institut National de Recherche et de Securité, France

  • Author: Jacobsen, Nicklas Raun

    National Research Centre for the Working Environment, Denmark

  • Author: Valentino, Sarah

    Institut National de Recherche et de Securité, France

  • Author: Wallin, Håkan

    National Institute of Occupational Health, Norway

  • Author: Bau, Sebastien

    Institut National de Recherche et de Securité, France

  • Author: Wolff, Henrik

    Finnish Institute of Occupational Health, Finland

  • Author: Sébillaud, Sylvie

    Institut National de Recherche et de Securité, France

  • Author: Lorcin, Mylène

    Institut National de Recherche et de Securité, France

  • Author: Grossmann, Stéphane

    Institut National de Recherche et de Securité, France

  • Author: Viton, Stéphane

    Institut National de Recherche et de Securité, France

  • Author: Nunge, Hervé

    Institut National de Recherche et de Securité, France

  • Author: Darne, Christian

    Institut National de Recherche et de Securité, France

  • Author: Vogel, Ulla Birgitte

    Department of Health Technology, Technical University of Denmark

  • Author: Cosnier, Frédéric

    Institut National de Recherche et de Securité, France

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Multi-walled carbon nanotubes (MWCNTs), which vary in length, diameter, functionalization and specific surface area, are used in diverse industrial processes. Since these nanomaterials have a high aspect ratio and are biopersistant in the lung, there is a need for a rapid identification of their potential health hazard. We assessed in Sprague-Dawley rats the pulmonary toxicity of two pristine MWCNTs (the "long and thick" NM-401 and the "short and thin" NM-403) following either intratracheal instillation or 4-week inhalation in order to gain insights into the predictability and intercomparability of the two methods. The deposited doses following inhalation were lower than the instilled doses. Both types of carbon nanotube induced pulmonary neutrophil influx using both exposure methods. This influx correlated with deposited surface area across MWCNT types and means of exposure at two different time points, 1-3 days and 28-30 days post-exposure. Increased levels of DNA damage were observed across doses and time points for both exposure methods, but no dose-response relationship was observed. Intratracheal instillation of NM-401 induced fibrosis at the highest dose while lower lung deposited doses obtained by inhalation did not induce such lung pathology. No fibrosis was observed following NM-403 exposure. When the deposited dose was taken into account, sub-acute inhalation and a single instillation of NM-401 and NM-403 produced very similar inflammation and DNA damage responses. Our data suggest that the dose-dependent inflammatory responses observed after intratracheal instillation and inhalation of MWCNTs are similar and were predicted by the deposited surface area.
Original languageEnglish
JournalTOXICOLOGY AND APPLIED PHARMACOLOGY
Volume375
Pages (from-to)17-31
ISSN0041-008X
DOIs
Publication statusPublished - 2019
CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • Carbon nanotubes, Inhalation, Intratracheal instillation, Lung inflammation, Rat

ID: 179294107