Pulmonary instillation of low doses of titanium dioxide nanoparticles in mice leads to particle retention and gene expression changes in the absence of inflammation

Mainul Husain; Anne Thoustrup Saber; Charles Guo; Nicklas R. Jacobsen; Keld Almand Jensen; Carole L. Yauk; Andrew Richard Williams; Ulla Birgitte Vogel; Hakan Wallin; Sabina Halappanavar

doi:10.1016/j.taap.2013.03.018

Pulmonary instillation of low doses of titanium dioxide nanoparticles in mice leads to particle retention and gene expression changes in the absence of inflammation

Mainul Husain, Anne Thoustrup Saber, Charles Guo, Nicklas R. Jacobsen, Keld Almand Jensen, Carole L. Yauk, Andrew Richard Williams, Ulla Birgitte Vogel, Hakan Wallin, Sabina Halappanavar

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Abstract

We investigated gene expression, protein synthesis, and particle retention in mouse lungs following intratracheal instillation of varying doses of nano-sized titanium dioxide (nano-TiO2). Female C57BL/6 mice were exposed to rutile nano-TiO2 via single intratracheal instillations of 18, 54, and 162μg/mouse. Mice were sampled 1, 3, and 28days post-exposure. The deposition of nano-TiO2 in the lungs was assessed using nanoscale hyperspectral microscopy. Biological responses in the pulmonary system were analyzed using DNA microarrays, pathway-specific real-time RT-PCR (qPCR), gene-specific qPCR arrays, and tissue protein ELISA. Hyperspectral mapping showed dose-dependent retention of nano-TiO2 in the lungs up to 28days post-instillation. DNA microarray analysis revealed approximately 3000 genes that were altered across all treatment groups (±1.3 fold; p

Original language	English
Journal	Toxicology and Applied Pharmacology
Volume	269
Issue number	3
Pages (from-to)	250-262
ISSN	0041-008X
DOIs	https://doi.org/10.1016/j.taap.2013.03.018
Publication status	Published - 2013

Keywords

Hyperspectral microscopy
Gene expression
Inflammation
Muscle contraction
Calcium homeostasis
Tissue particle retention

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Husain, M., Saber, A. T., Guo, C., Jacobsen, N. R., Jensen, K. A., Yauk, C. L., Williams, A. R., Vogel, U. B., Wallin, H., & Halappanavar, S. (2013). Pulmonary instillation of low doses of titanium dioxide nanoparticles in mice leads to particle retention and gene expression changes in the absence of inflammation. Toxicology and Applied Pharmacology, 269(3), 250-262. https://doi.org/10.1016/j.taap.2013.03.018

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title = "Pulmonary instillation of low doses of titanium dioxide nanoparticles in mice leads to particle retention and gene expression changes in the absence of inflammation",

abstract = "We investigated gene expression, protein synthesis, and particle retention in mouse lungs following intratracheal instillation of varying doses of nano-sized titanium dioxide (nano-TiO2). Female C57BL/6 mice were exposed to rutile nano-TiO2 via single intratracheal instillations of 18, 54, and 162μg/mouse. Mice were sampled 1, 3, and 28days post-exposure. The deposition of nano-TiO2 in the lungs was assessed using nanoscale hyperspectral microscopy. Biological responses in the pulmonary system were analyzed using DNA microarrays, pathway-specific real-time RT-PCR (qPCR), gene-specific qPCR arrays, and tissue protein ELISA. Hyperspectral mapping showed dose-dependent retention of nano-TiO2 in the lungs up to 28days post-instillation. DNA microarray analysis revealed approximately 3000 genes that were altered across all treatment groups (±1.3 fold; p",

keywords = "Hyperspectral microscopy, Gene expression, Inflammation, Muscle contraction, Calcium homeostasis, Tissue particle retention",

author = "Mainul Husain and Saber, {Anne Thoustrup} and Charles Guo and Jacobsen, {Nicklas R.} and Jensen, {Keld Almand} and Yauk, {Carole L.} and Williams, {Andrew Richard} and Vogel, {Ulla Birgitte} and Hakan Wallin and Sabina Halappanavar",

year = "2013",

doi = "10.1016/j.taap.2013.03.018",

language = "English",

volume = "269",

pages = "250--262",

journal = "Toxicology and Applied Pharmacology",

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Husain, M, Saber, AT, Guo, C, Jacobsen, NR, Jensen, KA, Yauk, CL, Williams, AR, Vogel, UB, Wallin, H & Halappanavar, S 2013, 'Pulmonary instillation of low doses of titanium dioxide nanoparticles in mice leads to particle retention and gene expression changes in the absence of inflammation', Toxicology and Applied Pharmacology, vol. 269, no. 3, pp. 250-262. https://doi.org/10.1016/j.taap.2013.03.018

Pulmonary instillation of low doses of titanium dioxide nanoparticles in mice leads to particle retention and gene expression changes in the absence of inflammation. / Husain, Mainul; Saber, Anne Thoustrup; Guo, Charles et al.
In: Toxicology and Applied Pharmacology, Vol. 269, No. 3, 2013, p. 250-262.

Research output: Contribution to journal › Journal article › Research › peer-review

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T1 - Pulmonary instillation of low doses of titanium dioxide nanoparticles in mice leads to particle retention and gene expression changes in the absence of inflammation

AU - Husain, Mainul

AU - Saber, Anne Thoustrup

AU - Guo, Charles

AU - Jacobsen, Nicklas R.

AU - Jensen, Keld Almand

AU - Yauk, Carole L.

AU - Williams, Andrew Richard

AU - Vogel, Ulla Birgitte

AU - Wallin, Hakan

AU - Halappanavar, Sabina

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AB - We investigated gene expression, protein synthesis, and particle retention in mouse lungs following intratracheal instillation of varying doses of nano-sized titanium dioxide (nano-TiO2). Female C57BL/6 mice were exposed to rutile nano-TiO2 via single intratracheal instillations of 18, 54, and 162μg/mouse. Mice were sampled 1, 3, and 28days post-exposure. The deposition of nano-TiO2 in the lungs was assessed using nanoscale hyperspectral microscopy. Biological responses in the pulmonary system were analyzed using DNA microarrays, pathway-specific real-time RT-PCR (qPCR), gene-specific qPCR arrays, and tissue protein ELISA. Hyperspectral mapping showed dose-dependent retention of nano-TiO2 in the lungs up to 28days post-instillation. DNA microarray analysis revealed approximately 3000 genes that were altered across all treatment groups (±1.3 fold; p

KW - Hyperspectral microscopy

KW - Gene expression

KW - Inflammation

KW - Muscle contraction

KW - Calcium homeostasis

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JO - Toxicology and Applied Pharmacology

JF - Toxicology and Applied Pharmacology

IS - 3

ER -

Pulmonary instillation of low doses of titanium dioxide nanoparticles in mice leads to particle retention and gene expression changes in the absence of inflammation

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Keywords

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