Pulmonary effects of nanofibrillated celluloses in mice suggest that carboxylation lowers the inflammatory and acute phase responses

Research output: Contribution to journalJournal article – Annual report year: 2019Researchpeer-review

  • Author: Hadrup, Niels

    National Research Centre for the Working Environment, Denmark

  • Author: Knudsen, Kristina Bram

    National Research Centre for the Working Environment, Denmark

  • Author: Berthing, Trine

    National Research Centre for the Working Environment, Denmark

  • Author: Wolff, Henrik

    Finnish Institute of Occupational Health, Finland

  • Author: Bengtson, Stefan

    National Research Centre for the Working Environment, Denmark

  • Author: Kofoed, Steen Christian

    University of Copenhagen, Denmark

  • Author: Espersen, Roall

    University of Copenhagen, Denmark

  • Author: Højgaard, Casper

    University of Copenhagen, Denmark

  • Author: Winther, Jakob Rahr

    University of Copenhagen, Denmark

  • Author: Willemoes, Martin

    University of Copenhagen, Denmark

  • Author: Wedin, Irene

    Stora Enso, Finland

  • Author: Nuopponen, Markus

    UPM Kymmene, Finland

  • Author: Alenius, Harri

    University of Helsinki, Finland

  • Author: Norppa, Hannu

    Finnish Institute of Occupational Health, Finland

  • Author: Wallin, Håkan

    National Institute of Occupational Health, Norway

  • Author: Vogel, Ulla Birgitte

    Department of Health Technology, Technical University of Denmark

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We studied if the pulmonary and systemic toxicity of nanofibrillated celluloses can be reduced by carboxylation. Nanofibrillated celluloses administered at 6 or 18 μg to mice by intratracheal instillation were: 1) FINE NFC, 2–20 μm in length, 2–15 nm in width, 2) AS (−COOH), carboxylated, 0.5–10 μm in length, 4–10 nm in width, containing the biocide BIM MC4901 and 3) BIOCID FINE NFC: as (1) but containing BIM MC4901. FINE NFC administration increased neutrophil influx in BAL and induced SAA3 in plasma. AS (−COOH) produced lower neutrophil influx and systemic SAA3 levels than FINE NFC. Results obtained with BIOCID FINE NFC suggested that BIM MC4901 biocide did not explain the lowered response. Increased DNA damage levels were observed across materials, doses and time points. In conclusion, carboxylation of nanofibrillated cellulose was associated with reduced pulmonary and systemic toxicity, suggesting involvement of OH groups in the inflammatory and acute phase responses.
Original languageEnglish
JournalENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
Volume66
Pages (from-to)116-125
ISSN1382-6689
DOIs
Publication statusPublished - 2019
CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • Genotoxicity, Nanocellulose, Nanomaterial, Nanoparticle, Neutrophils, Serum amyloid A

ID: 185052176