Pulmonary effects of nanofibrillated celluloses in mice suggest that carboxylation lowers the inflammatory and acute phase responses

Niels Hadrup, Kristina Bram Knudsen, Trine Berthing, Henrik Wolff, Stefan Bengtson, Christian Kofoed, Roall Espersen, Casper Højgaard, Jakob Rahr Winther, Martin Willemoes, Irene Wedin, Markus Nuopponen, Harri Alenius, Hannu Norppa, Håkan Wallin, Ulla Birgitte Vogel*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We studied if the pulmonary and systemic toxicity of nanofibrillated celluloses can be reduced by carboxylation. Nanofibrillated celluloses administered at 6 or 18 μg to mice by intratracheal instillation were: 1) FINE NFC, 2–20 μm in length, 2–15 nm in width, 2) AS (−COOH), carboxylated, 0.5–10 μm in length, 4–10 nm in width, containing the biocide BIM MC4901 and 3) BIOCID FINE NFC: as (1) but containing BIM MC4901. FINE NFC administration increased neutrophil influx in BAL and induced SAA3 in plasma. AS (−COOH) produced lower neutrophil influx and systemic SAA3 levels than FINE NFC. Results obtained with BIOCID FINE NFC suggested that BIM MC4901 biocide did not explain the lowered response. Increased DNA damage levels were observed across materials, doses and time points. In conclusion, carboxylation of nanofibrillated cellulose was associated with reduced pulmonary and systemic toxicity, suggesting involvement of OH groups in the inflammatory and acute phase responses.
Original languageEnglish
JournalENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
Volume66
Pages (from-to)116-125
ISSN1382-6689
DOIs
Publication statusPublished - 2019

Keywords

  • Genotoxicity
  • Nanocellulose
  • Nanomaterial
  • Nanoparticle
  • Neutrophils
  • Serum amyloid A

Cite this

Hadrup, Niels ; Knudsen, Kristina Bram ; Berthing, Trine ; Wolff, Henrik ; Bengtson, Stefan ; Kofoed, Christian ; Espersen, Roall ; Højgaard, Casper ; Winther, Jakob Rahr ; Willemoes, Martin ; Wedin, Irene ; Nuopponen, Markus ; Alenius, Harri ; Norppa, Hannu ; Wallin, Håkan ; Vogel, Ulla Birgitte. / Pulmonary effects of nanofibrillated celluloses in mice suggest that carboxylation lowers the inflammatory and acute phase responses. In: ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY. 2019 ; Vol. 66. pp. 116-125.
@article{83e7bb04fcbb4985a67cf239dbe46a64,
title = "Pulmonary effects of nanofibrillated celluloses in mice suggest that carboxylation lowers the inflammatory and acute phase responses",
abstract = "We studied if the pulmonary and systemic toxicity of nanofibrillated celluloses can be reduced by carboxylation. Nanofibrillated celluloses administered at 6 or 18 μg to mice by intratracheal instillation were: 1) FINE NFC, 2–20 μm in length, 2–15 nm in width, 2) AS (−COOH), carboxylated, 0.5–10 μm in length, 4–10 nm in width, containing the biocide BIM MC4901 and 3) BIOCID FINE NFC: as (1) but containing BIM MC4901. FINE NFC administration increased neutrophil influx in BAL and induced SAA3 in plasma. AS (−COOH) produced lower neutrophil influx and systemic SAA3 levels than FINE NFC. Results obtained with BIOCID FINE NFC suggested that BIM MC4901 biocide did not explain the lowered response. Increased DNA damage levels were observed across materials, doses and time points. In conclusion, carboxylation of nanofibrillated cellulose was associated with reduced pulmonary and systemic toxicity, suggesting involvement of OH groups in the inflammatory and acute phase responses.",
keywords = "Genotoxicity, Nanocellulose, Nanomaterial, Nanoparticle, Neutrophils, Serum amyloid A",
author = "Niels Hadrup and Knudsen, {Kristina Bram} and Trine Berthing and Henrik Wolff and Stefan Bengtson and Christian Kofoed and Roall Espersen and Casper H{\o}jgaard and Winther, {Jakob Rahr} and Martin Willemoes and Irene Wedin and Markus Nuopponen and Harri Alenius and Hannu Norppa and H{\aa}kan Wallin and Vogel, {Ulla Birgitte}",
year = "2019",
doi = "10.1016/j.etap.2019.01.003",
language = "English",
volume = "66",
pages = "116--125",
journal = "Environmental Toxicology and Pharmacology",
issn = "1382-6689",
publisher = "Elsevier",

}

Hadrup, N, Knudsen, KB, Berthing, T, Wolff, H, Bengtson, S, Kofoed, C, Espersen, R, Højgaard, C, Winther, JR, Willemoes, M, Wedin, I, Nuopponen, M, Alenius, H, Norppa, H, Wallin, H & Vogel, UB 2019, 'Pulmonary effects of nanofibrillated celluloses in mice suggest that carboxylation lowers the inflammatory and acute phase responses', ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, vol. 66, pp. 116-125. https://doi.org/10.1016/j.etap.2019.01.003

Pulmonary effects of nanofibrillated celluloses in mice suggest that carboxylation lowers the inflammatory and acute phase responses. / Hadrup, Niels; Knudsen, Kristina Bram; Berthing, Trine; Wolff, Henrik; Bengtson, Stefan; Kofoed, Christian; Espersen, Roall; Højgaard, Casper; Winther, Jakob Rahr; Willemoes, Martin; Wedin, Irene; Nuopponen, Markus; Alenius, Harri; Norppa, Hannu; Wallin, Håkan; Vogel, Ulla Birgitte.

In: ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, Vol. 66, 2019, p. 116-125.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Pulmonary effects of nanofibrillated celluloses in mice suggest that carboxylation lowers the inflammatory and acute phase responses

AU - Hadrup, Niels

AU - Knudsen, Kristina Bram

AU - Berthing, Trine

AU - Wolff, Henrik

AU - Bengtson, Stefan

AU - Kofoed, Christian

AU - Espersen, Roall

AU - Højgaard, Casper

AU - Winther, Jakob Rahr

AU - Willemoes, Martin

AU - Wedin, Irene

AU - Nuopponen, Markus

AU - Alenius, Harri

AU - Norppa, Hannu

AU - Wallin, Håkan

AU - Vogel, Ulla Birgitte

PY - 2019

Y1 - 2019

N2 - We studied if the pulmonary and systemic toxicity of nanofibrillated celluloses can be reduced by carboxylation. Nanofibrillated celluloses administered at 6 or 18 μg to mice by intratracheal instillation were: 1) FINE NFC, 2–20 μm in length, 2–15 nm in width, 2) AS (−COOH), carboxylated, 0.5–10 μm in length, 4–10 nm in width, containing the biocide BIM MC4901 and 3) BIOCID FINE NFC: as (1) but containing BIM MC4901. FINE NFC administration increased neutrophil influx in BAL and induced SAA3 in plasma. AS (−COOH) produced lower neutrophil influx and systemic SAA3 levels than FINE NFC. Results obtained with BIOCID FINE NFC suggested that BIM MC4901 biocide did not explain the lowered response. Increased DNA damage levels were observed across materials, doses and time points. In conclusion, carboxylation of nanofibrillated cellulose was associated with reduced pulmonary and systemic toxicity, suggesting involvement of OH groups in the inflammatory and acute phase responses.

AB - We studied if the pulmonary and systemic toxicity of nanofibrillated celluloses can be reduced by carboxylation. Nanofibrillated celluloses administered at 6 or 18 μg to mice by intratracheal instillation were: 1) FINE NFC, 2–20 μm in length, 2–15 nm in width, 2) AS (−COOH), carboxylated, 0.5–10 μm in length, 4–10 nm in width, containing the biocide BIM MC4901 and 3) BIOCID FINE NFC: as (1) but containing BIM MC4901. FINE NFC administration increased neutrophil influx in BAL and induced SAA3 in plasma. AS (−COOH) produced lower neutrophil influx and systemic SAA3 levels than FINE NFC. Results obtained with BIOCID FINE NFC suggested that BIM MC4901 biocide did not explain the lowered response. Increased DNA damage levels were observed across materials, doses and time points. In conclusion, carboxylation of nanofibrillated cellulose was associated with reduced pulmonary and systemic toxicity, suggesting involvement of OH groups in the inflammatory and acute phase responses.

KW - Genotoxicity

KW - Nanocellulose

KW - Nanomaterial

KW - Nanoparticle

KW - Neutrophils

KW - Serum amyloid A

U2 - 10.1016/j.etap.2019.01.003

DO - 10.1016/j.etap.2019.01.003

M3 - Journal article

C2 - 30665014

VL - 66

SP - 116

EP - 125

JO - Environmental Toxicology and Pharmacology

JF - Environmental Toxicology and Pharmacology

SN - 1382-6689

ER -