Psoriasis patients with basal cell carcinoma have more repair-mediated DNA strand-breaks after UVC damage in lymphocytes than psoriasis patients without basal cell carcinoma

Peter Møller*, Håkan Wallin, Marianne Dybdahl, Gerda Frentz, Bjørn A. Nexø

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We have investigated the formation of strand-breaks following UVC irradiation in lymphocytes from psoriasis patients with or without basal cell carcinoma (BCC). Isolated lymphocytes were irradiated with UVC light at a dose of 3.6 J/m2, and the level of DNA strand-breaks were measured 25 min after the irradiation by the alkaline comet assay. The generation of strand-breaks following UVC irradiation indicates DNA-repair-mediated incisions, as UVC light does not generate strand-breaks per se. We found that psoriasis patients with BCC had more DNA-repair incisions than non-cancer patients. The incision level correlated to two polymorphisms of the XPD gene. At present, it is not clear if the association is a primary effect that is related to differences of the XPD protein. Genes encoding for other repair proteins, namely XRCC1, ERCC1, and LIG1 are located close to the XPD gene, and it is possible that the association is due to a cosegregation with a polymorphism in one of these genes. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
JournalCancer Letters
Volume151
Issue number2
Pages (from-to)187-192
Number of pages6
ISSN0304-3835
DOIs
Publication statusPublished - 14 Apr 2000
Externally publishedYes

Keywords

  • Basal cell carcinoma
  • Comet assay
  • DNA-repair activity
  • Psoriasis
  • XPD gene

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