Pseudoalteromonas haloplanktis TAC125 produces 4-hydroxybenzoic acid that induces pyroptosis in human A459 lung adenocarcinoma cells

Filomena Sannino, Clementina Sansone, Christian Galasso, Sara Kildgaard, Pietro Tedesco, Renato Fani, Gennaro Marino, Donatella de Pascale, Adrianna Ianora, Ermenegilda Parrilli, Thomas Ostenfeld Larsen, Giovanna Romano, Maria Luisa Tutino*

*Corresponding author for this work

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Abstract

In order to exploit the rich reservoir of marine cold-adapted bacteria as a source of bioactive metabolites, ethyl acetate crude extracts of thirteen polar marine bacteria were tested for their antiproliferative activity on A549 lung epithelial cancer cells. The crude extract from Pseudoalteromonas haloplanktis TAC125 was the most active in inhibiting cell proliferation. Extensive bioassay-guided purification and mass spectrometric characterization allowed the identification of 4-hydroxybenzoic acid (4-HBA) as the molecule responsible for this bioactivity. We further demonstrate that 4-HBA inhibits A549 cancer cell proliferation with an IC50 value
Original languageEnglish
Article number1190
JournalScientific Reports
Volume8
Issue number1
Number of pages10
ISSN2045-2322
DOIs
Publication statusPublished - 2018

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