Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids

Anne Zebitz Eriksen; Rasmus Møller; Bar Makovoz; Benjamin R. tenOever; Timothy A. Blenkinsop

doi:10.1016/j.xpro.2022.101383

Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids

Anne Zebitz Eriksen^*
, Rasmus Møller
, Bar Makovoz
, Benjamin R. tenOever
, Timothy A. Blenkinsop^*

^*Corresponding author for this work

Department of Health Technology

New York University
Icahn School of Medicine at Mount Sinai

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Here, we describe a series of protocols detailing the steps for evaluating SARS-CoV-2 infection in models of the human eye. Included are protocols for whole eye organoid differentiation, SARS-CoV-2 infection, and processing organoids for single-cell RNA sequencing. Additional protocols describe how to dissect and culture adult human ocular cells from cadaver donor eyes and how to compare infection of SARS-CoV-2 and the presence of SARS-CoV-2 entry factors using qPCR, immunofluorescence, and plaque assays. For complete details on the use and execution of this protocol, please refer to Eriksen et al. (2021).

Original language	English
Article number	101383
Journal	STAR Protocols
Volume	3
Issue number	2
Number of pages	23
ISSN	2666-1667
DOIs	https://doi.org/10.1016/j.xpro.2022.101383
Publication status	Published - 2022

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title = "Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids",

abstract = "Here, we describe a series of protocols detailing the steps for evaluating SARS-CoV-2 infection in models of the human eye. Included are protocols for whole eye organoid differentiation, SARS-CoV-2 infection, and processing organoids for single-cell RNA sequencing. Additional protocols describe how to dissect and culture adult human ocular cells from cadaver donor eyes and how to compare infection of SARS-CoV-2 and the presence of SARS-CoV-2 entry factors using qPCR, immunofluorescence, and plaque assays. For complete details on the use and execution of this protocol, please refer to Eriksen et al. (2021).",

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AU - Eriksen, Anne Zebitz

AU - Møller, Rasmus

AU - Makovoz, Bar

AU - tenOever, Benjamin R.

AU - Blenkinsop, Timothy A.

PY - 2022

Y1 - 2022

N2 - Here, we describe a series of protocols detailing the steps for evaluating SARS-CoV-2 infection in models of the human eye. Included are protocols for whole eye organoid differentiation, SARS-CoV-2 infection, and processing organoids for single-cell RNA sequencing. Additional protocols describe how to dissect and culture adult human ocular cells from cadaver donor eyes and how to compare infection of SARS-CoV-2 and the presence of SARS-CoV-2 entry factors using qPCR, immunofluorescence, and plaque assays. For complete details on the use and execution of this protocol, please refer to Eriksen et al. (2021).

AB - Here, we describe a series of protocols detailing the steps for evaluating SARS-CoV-2 infection in models of the human eye. Included are protocols for whole eye organoid differentiation, SARS-CoV-2 infection, and processing organoids for single-cell RNA sequencing. Additional protocols describe how to dissect and culture adult human ocular cells from cadaver donor eyes and how to compare infection of SARS-CoV-2 and the presence of SARS-CoV-2 entry factors using qPCR, immunofluorescence, and plaque assays. For complete details on the use and execution of this protocol, please refer to Eriksen et al. (2021).

U2 - 10.1016/j.xpro.2022.101383

DO - 10.1016/j.xpro.2022.101383

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JO - STAR Protocols

JF - STAR Protocols

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M1 - 101383

ER -

Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids

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