TY - JOUR
T1 - Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids
AU - Eriksen, Anne Zebitz
AU - Møller, Rasmus
AU - Makovoz, Bar
AU - tenOever, Benjamin R.
AU - Blenkinsop, Timothy A.
PY - 2022
Y1 - 2022
N2 - Here, we describe a series of protocols detailing the steps for evaluating SARS-CoV-2 infection in models of the human eye. Included are protocols for whole eye organoid differentiation, SARS-CoV-2 infection, and processing organoids for single-cell RNA sequencing. Additional protocols describe how to dissect and culture adult human ocular cells from cadaver donor eyes and how to compare infection of SARS-CoV-2 and the presence of SARS-CoV-2 entry factors using qPCR, immunofluorescence, and plaque assays. For complete details on the use and execution of this protocol, please refer to Eriksen et al. (2021).
AB - Here, we describe a series of protocols detailing the steps for evaluating SARS-CoV-2 infection in models of the human eye. Included are protocols for whole eye organoid differentiation, SARS-CoV-2 infection, and processing organoids for single-cell RNA sequencing. Additional protocols describe how to dissect and culture adult human ocular cells from cadaver donor eyes and how to compare infection of SARS-CoV-2 and the presence of SARS-CoV-2 entry factors using qPCR, immunofluorescence, and plaque assays. For complete details on the use and execution of this protocol, please refer to Eriksen et al. (2021).
U2 - 10.1016/j.xpro.2022.101383
DO - 10.1016/j.xpro.2022.101383
M3 - Journal article
C2 - 35664254
SN - 2666-1667
VL - 3
JO - STAR Protocols
JF - STAR Protocols
IS - 2
M1 - 101383
ER -