Proteome analysis demonstrates profound alterations in human dendritic cell nature by TX527, an analogue of vitamin D

G. B. Ferreira, E. van Etten, K. Lage, Daniel Aaen Hansen, Yves Moreau, Christopher Workman, M. Waer, A. Verstuyf, E. Waelkens, L. Overbergh, C. Mathieu

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Structural analogues of vitamin D have been put forward as therapeutic agents able to exploit the immunomodulatory effects of vitamin D, without its undesired calcemic side effects. We have demonstrated that TX527 affects dendritic cell (DC) maturation in vitro, resulting in the generation of a tolerogenic cell. In the present study, we aimed to explore the global protein changes induced by the analogue in immature DC (iDC) and mature human DC and to correlate them with alterations in DC morphology and function. Human CD14(+) monocytes were differentiated toward iDC or mature DCs, in the presence or absence of TX527 (10(-8) M) (n = 4). Protein samples were separated into two different pH ranges (pH4-7 and 6-9), analyzed by 2-D DIGE and differentially expressed spots (p
    Original languageEnglish
    JournalProteomics
    Volume9
    Issue number14
    Pages (from-to)3752-3764
    ISSN1615-9853
    DOIs
    Publication statusPublished - 2009

    Keywords

    • 2-D DIGE
    • Network
    • Vitamin D analogue
    • Dendritic cell
    • Immunotolerance

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