Proteinaceous methanotrophs for feed additive using biowaste as carbon and nutrients source

Panagiotis Tsapekos*, Xinyu Zhu, Evangelos Pallis, Irini Angelidaki

*Corresponding author for this work

    Research output: Contribution to journalJournal articleResearchpeer-review

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    Abstract

    To achieve a sustainable production of food and feed production, inexpensive carbon and nutrient sources are needed. In the present study, biologically upgraded biogas is coupled with electrochemically extracted nitrogen from digested biowaste to cultivate mixed methanotrophs as protein source. Results showed that an increase from less than 5 μgCu2+/L to 100 μgCu2+/L increased the biomass production by 41%. Microbial analysis revealed that the dominated Methylomonas spp. followed by Methylophilus spp. created a specialized community for high CH4 assimilation. Moreover, duplicate semi-continuous fermenters run for 120 days validating the efficiency of alternative carbon and nitrogen feedstocks at long-term operation. As for dry cell weight (DCW) production, more than 2.5 g-DCW/L were produced using biologically upgraded biogas and electrochemically extracted nitrogen. Furthermore, the protein content and amino acid profile (>50% of DCW) demonstrated that the microbial biomass pose the characteristics to be used as animal feed additive.

    Original languageEnglish
    Article number123646
    JournalBioresource Technology
    Volume313
    ISSN0960-8524
    DOIs
    Publication statusPublished - 2020

    Keywords

    • 16S rRNA
    • Biomethane
    • Digested biowaste
    • Methanotrophs
    • Microbial protein

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