Protein-induced changes during the maturation process of human dendritic cells: A 2-D DIGE approach

Gb Ferreira, L Overbergh, Kasper Lage Hansen, W D'Hertog, Daniel Aaen Hansen, M Maris, Y Moreau, Christopher Workman, E Waelkens, C Mathieu

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    Abstract

    Dendritic cells (DCs) are unique antigen presenting cells, which upon maturation change from a specialized antigen-capturing cell towards a professional antigen presenting cells. In this study, a 2-D DIGE analysis of immature and mature DCs was performed, to identify proteins changing in expression upon maturation. The protein expression profile of immature and mature DCs, derived from CD14+ peripheral blood monocytes was investigated using two pH ranges (pH 4-7 and 6-9) (n = 4). Ninety one differentially expressed spots (p
    Original languageEnglish
    JournalProteomics - Clinical Applications
    Volume2
    Issue number9
    Pages (from-to)1349-1360
    ISSN1862-8346
    DOIs
    Publication statusPublished - 2008

    Cite this

    Ferreira, G., Overbergh, L., Hansen, K. L., D'Hertog, W., Hansen, D. A., Maris, M., Moreau, Y., Workman, C., Waelkens, E., & Mathieu, C. (2008). Protein-induced changes during the maturation process of human dendritic cells: A 2-D DIGE approach. Proteomics - Clinical Applications, 2(9), 1349-1360. https://doi.org/10.1002/prca.200800110