Abstract
Rainbow trout fingerlings were immunized by intramuscular injection of a plasmid DNA vector encoding the viral haemorrhagic septicaemia virus (VHSV) glycoprotein (G) or nucleocapsid protein (N) genes under the control of a cytomegalovirus promoter. Challenge with VHSV 52 days later demonstrated that both viral genes, and the G gene in particular, were able to induce protective immunity against VHS. In contrast to sera taken from fish injected with the N gene, neutralizing antibody activity could be detected both before and after challenge in the sera of a major proportion of the fish injected with the G gene.
| Original language | English |
|---|---|
| Journal | Fish & shellfish immunology |
| Volume | 8 |
| Issue number | 4 |
| Pages (from-to) | 261-270 |
| ISSN | 1050-4648 |
| Publication status | Published - 1998 |
Keywords
- protection
- DNA vaccine
- Engtved virus
- antibody response
