Protective immunity to VHS in rainbow trout (Oncorhynchus mykiss, Walbaum) following DNA vaccination

Niels Lorenzen, Ellen Lorenzen, Katja Einer-Jensen, J. Heppell, T. Wu, H. Davis

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Rainbow trout fingerlings were immunized by intramuscular injection of a plasmid DNA vector encoding the viral haemorrhagic septicaemia virus (VHSV) glycoprotein (G) or nucleocapsid protein (N) genes under the control of a cytomegalovirus promoter. Challenge with VHSV 52 days later demonstrated that both viral genes, and the G gene in particular, were able to induce protective immunity against VHS. In contrast to sera taken from fish injected with the N gene, neutralizing antibody activity could be detected both before and after challenge in the sera of a major proportion of the fish injected with the G gene.
    Original languageEnglish
    JournalFish & shellfish immunology
    Volume8
    Issue number4
    Pages (from-to)261-270
    ISSN1050-4648
    Publication statusPublished - 1998

    Keywords

    • protection
    • DNA vaccine
    • Engtved virus
    • antibody response

    Cite this

    Lorenzen, N., Lorenzen, E., Einer-Jensen, K., Heppell, J., Wu, T., & Davis, H. (1998). Protective immunity to VHS in rainbow trout (Oncorhynchus mykiss, Walbaum) following DNA vaccination. Fish & shellfish immunology, 8(4), 261-270.