Protective immune responses in lawsonia intracellularis infections

Henriette Cordes, Ulla Riber, Torsten Boutrup, Tim Kåre Jensen, Lien Thi Minh Nguyen, Gregers Jungersen

    Research output: Contribution to conferenceConference abstract for conferenceResearch

    Abstract

    Lawsonia intracellularis is the cause of porcine proliferative enteropathy, one of the major causes of antibiotics usage in modern pig production. L. intracellularis is an obligate intracellular bacterium preferable infecting epithelial cells of pigs intestine. We have demonstrated earlier, that a primary L. intracellularis experimental infection in pigs protects against re-colonisation (re-infection) with a virulent L. intracellularis isolate. After re-infection the animals had reduced L. intracellularis colonisation of the intestinal mucosa compared to controls, no bacterial shedding and no increase in acute phase response after challenge with a pathogenic isolate. Here we show results from measurements of serology as well as cell-mediated immune responses from this experiment. We found that Lawsonia-specific IgA peaked in serum around day 17-24 after a primary infection in experimentally infected piglets where after it levelled off. There was no boost in this response after re-infection, but boosting was observed with serum IgG, resulting in an increasing IgG/IgA index. Local secretory IgA, on the other hand were low following a primary infection, probably due to age-related effects, but exhibited a high, but short-lasting peak after re-infection. Specific IFN responses were also measured using a whole blood IFN-γ assay. These were very high in challenge infected and re-infected animals as compared to controls. These specific immune responses may contribute to the explanation of mechanisms behind the observed protection against re-infection with L. intracellularis.
    Original languageEnglish
    Publication date2009
    Publication statusPublished - 2009
    Event8th ELSINORE MEETING : Prophylactic & Therapeutic Intervention in Host-Pathogen Interaction - Helsingør, Denmark
    Duration: 1 Jan 2009 → …
    Conference number: 8.

    Conference

    Conference8th ELSINORE MEETING : Prophylactic & Therapeutic Intervention in Host-Pathogen Interaction
    Number8.
    CityHelsingør, Denmark
    Period01/01/2009 → …

    Cite this

    Cordes, H., Riber, U., Boutrup, T., Jensen, T. K., Nguyen, L. T. M., & Jungersen, G. (2009). Protective immune responses in lawsonia intracellularis infections. Abstract from 8th ELSINORE MEETING : Prophylactic & Therapeutic Intervention in Host-Pathogen Interaction, Helsingør, Denmark, .