Protective effect of in-feed specific IgM towards Yersinia ruckeri in rainbow trout

Jiwan Kumar Chettri*, Azmi Al-Jubury, Marie B. Hansen, Allan Lihme, Inger Dalsgaard, Kurt Buchmann, Peter M. H. Heegaard

*Corresponding author for this work

Research output: Contribution to journalComment/debateResearchpeer-review

Abstract

Tightened regulations and an environmentally friendly approaches in fish production have greatly reduced the use of antibiotics but green solutions are continuously being explored. The use of functional feed may have a potential in the aquaculture sector in securing biomass and minimizing the loss from disease. In the present study, we tested the concept that blood from the fish slaughterhouse can be used for mass purification of specific antibodies which subsequently can be used for feeding fish and thereby confer protection against diseases. IgM was purified from serum from Yersinia ruckeri vaccinated rainbow trout and an IgM sandwich ELISA was developed for quantification of rainbow trout IgM. The purified IgM was encapsulated in alginate microparticles and top-coated in fish feed. IgM re-extracted from the alginate microparticles was shown to retain high reactivity towards Y. ruckeri antigens indicating that its bioactivity remained intact after encapsulation. IgM release from the alginate microparticles was only observed at high pH (pH 8.2) and minimal at low pH, indicating protection of IgM at low pH in the fish stomach during passage. In a feeding - challenge experiment (feeding 1 week before Y. ruckeri challenge and for two weeks following challenge), a statistically non-significant 10% lower mortality was observed in the high dose (400 μg IgM/fish/day fed over 3 weeks) group.
Original languageEnglish
JournalFish and Shellfish Immunology
Volume93
Pages (from-to)934-939
ISSN1050-4648
DOIs
Publication statusPublished - 2019

Keywords

  • Alginate encapsulation
  • IgM feeding
  • Passive immunization
  • Protection
  • Yersinia ruckeri

Cite this

Chettri, Jiwan Kumar ; Al-Jubury, Azmi ; Hansen, Marie B. ; Lihme, Allan ; Dalsgaard, Inger ; Buchmann, Kurt ; Heegaard, Peter M. H. / Protective effect of in-feed specific IgM towards Yersinia ruckeri in rainbow trout. In: Fish and Shellfish Immunology. 2019 ; Vol. 93. pp. 934-939.
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abstract = "Tightened regulations and an environmentally friendly approaches in fish production have greatly reduced the use of antibiotics but green solutions are continuously being explored. The use of functional feed may have a potential in the aquaculture sector in securing biomass and minimizing the loss from disease. In the present study, we tested the concept that blood from the fish slaughterhouse can be used for mass purification of specific antibodies which subsequently can be used for feeding fish and thereby confer protection against diseases. IgM was purified from serum from Yersinia ruckeri vaccinated rainbow trout and an IgM sandwich ELISA was developed for quantification of rainbow trout IgM. The purified IgM was encapsulated in alginate microparticles and top-coated in fish feed. IgM re-extracted from the alginate microparticles was shown to retain high reactivity towards Y. ruckeri antigens indicating that its bioactivity remained intact after encapsulation. IgM release from the alginate microparticles was only observed at high pH (pH 8.2) and minimal at low pH, indicating protection of IgM at low pH in the fish stomach during passage. In a feeding - challenge experiment (feeding 1 week before Y. ruckeri challenge and for two weeks following challenge), a statistically non-significant 10{\%} lower mortality was observed in the high dose (400 μg IgM/fish/day fed over 3 weeks) group.",
keywords = "Alginate encapsulation, IgM feeding, Passive immunization, Protection, Yersinia ruckeri",
author = "Chettri, {Jiwan Kumar} and Azmi Al-Jubury and Hansen, {Marie B.} and Allan Lihme and Inger Dalsgaard and Kurt Buchmann and Heegaard, {Peter M. H.}",
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Protective effect of in-feed specific IgM towards Yersinia ruckeri in rainbow trout. / Chettri, Jiwan Kumar; Al-Jubury, Azmi; Hansen, Marie B.; Lihme, Allan; Dalsgaard, Inger; Buchmann, Kurt; Heegaard, Peter M. H.

In: Fish and Shellfish Immunology, Vol. 93, 2019, p. 934-939.

Research output: Contribution to journalComment/debateResearchpeer-review

TY - JOUR

T1 - Protective effect of in-feed specific IgM towards Yersinia ruckeri in rainbow trout

AU - Chettri, Jiwan Kumar

AU - Al-Jubury, Azmi

AU - Hansen, Marie B.

AU - Lihme, Allan

AU - Dalsgaard, Inger

AU - Buchmann, Kurt

AU - Heegaard, Peter M. H.

PY - 2019

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N2 - Tightened regulations and an environmentally friendly approaches in fish production have greatly reduced the use of antibiotics but green solutions are continuously being explored. The use of functional feed may have a potential in the aquaculture sector in securing biomass and minimizing the loss from disease. In the present study, we tested the concept that blood from the fish slaughterhouse can be used for mass purification of specific antibodies which subsequently can be used for feeding fish and thereby confer protection against diseases. IgM was purified from serum from Yersinia ruckeri vaccinated rainbow trout and an IgM sandwich ELISA was developed for quantification of rainbow trout IgM. The purified IgM was encapsulated in alginate microparticles and top-coated in fish feed. IgM re-extracted from the alginate microparticles was shown to retain high reactivity towards Y. ruckeri antigens indicating that its bioactivity remained intact after encapsulation. IgM release from the alginate microparticles was only observed at high pH (pH 8.2) and minimal at low pH, indicating protection of IgM at low pH in the fish stomach during passage. In a feeding - challenge experiment (feeding 1 week before Y. ruckeri challenge and for two weeks following challenge), a statistically non-significant 10% lower mortality was observed in the high dose (400 μg IgM/fish/day fed over 3 weeks) group.

AB - Tightened regulations and an environmentally friendly approaches in fish production have greatly reduced the use of antibiotics but green solutions are continuously being explored. The use of functional feed may have a potential in the aquaculture sector in securing biomass and minimizing the loss from disease. In the present study, we tested the concept that blood from the fish slaughterhouse can be used for mass purification of specific antibodies which subsequently can be used for feeding fish and thereby confer protection against diseases. IgM was purified from serum from Yersinia ruckeri vaccinated rainbow trout and an IgM sandwich ELISA was developed for quantification of rainbow trout IgM. The purified IgM was encapsulated in alginate microparticles and top-coated in fish feed. IgM re-extracted from the alginate microparticles was shown to retain high reactivity towards Y. ruckeri antigens indicating that its bioactivity remained intact after encapsulation. IgM release from the alginate microparticles was only observed at high pH (pH 8.2) and minimal at low pH, indicating protection of IgM at low pH in the fish stomach during passage. In a feeding - challenge experiment (feeding 1 week before Y. ruckeri challenge and for two weeks following challenge), a statistically non-significant 10% lower mortality was observed in the high dose (400 μg IgM/fish/day fed over 3 weeks) group.

KW - Alginate encapsulation

KW - IgM feeding

KW - Passive immunization

KW - Protection

KW - Yersinia ruckeri

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