Promoting and Impeding Effects of Lytic Polysaccharide Monooxygenases on Glycoside Hydrolase Activity

Malene Billeskov Keller, Silke Flindt Badino, Benedikt Möllers Blossom, Brett McBrayer, Kim Borch, Peter Westh*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review


Lytic polysaccharide monooxygenases (LPMOs) have attracted attention due to their ability to boost cellulolytic enzyme cocktails for application in biorefineries. However, the interplay between LPMOs and individual glycoside hydrolases remains poorly understood. We investigated how the activity of two cellobiohydrolases (Cel7A and Cel6A) and an endoglucanase (Cel7B) from Trichoderma reesei were affected by a C1-oxidizing LPMO from Thielavia terrestris (TtAA9). We quantified products from a mixture of LPMO and glycoside hydrolase and estimated separate contributions of products by each of the enzymes. Hereby, we assessed if an observed synergy reflected a promotion of the activity of hydrolase, LPMO, or both. We consistently found that TtAA9 affected the investigated hydrolases differently. It strongly impeded the turnover of the reducing end cellobiohydrolase, TrCel7A, moderately promoted the turnover of the nonreducing end cellobiohydrolase TrCel6A, and promoted the turnover of the endoglucanase, TrCel7B up to 5-fold. The promoting effect on the endoglucanase increased with hydrolysis extent, indicating that the promoting effect became more important as the recalcitrance of the substrate increased. Experiments with mixtures containing multiple glycoside hydrolases suggested that the LPMO primarily promoted the activity of the endoglucanase, whereas promotion of TrCel6A was secondary.
Original languageEnglish
JournalACS Sustainable Chemistry and Engineering
Issue number37
Pages (from-to)14117-14126
Number of pages10
Publication statusPublished - 2020


  • Lytic polysaccharide monooxygenases (LPMO)
  • Glycoside hydrolases (GH)
  • Synergism
  • Endoglucanases
  • Cellulase cocktails
  • Lignocellulose
  • Biorefinery


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