Progress in cyclodextrins as important molecules regulating catalytic processes of glycoside hydrolases

Xiaoxiao Li, Zhengyu Jin, Yuxiang Bai*, Birte Svensson

*Corresponding author for this work

Research output: Contribution to journalReviewpeer-review


Cyclodextrins (CDs) are important starch derivatives and commonly comprise α-, β-, and γ-CDs. Their hydrophilic surface and hydrophobic inner cavity enable regulation of enzyme catalysis through direct or indirect interactions. Clarifying interactions between CDs and enzyme is of great value for enzyme screening, mechanism exploration, regulation of catalysis, and applications. We summarize the interactions between CDs and glycoside hydrolases (GHs) according to two aspects: 1) CD as products, substrates, inhibitors and activators of enzymes, directly affecting the reaction process; 2) CDs indirectly affecting the enzymatic reaction by solubilizing substrates, relieving substrate/product inhibition, increasing recombinant enzyme production and storage stability, isolating and purifying enzymes, and serving as ligands in crystal structure to identify functional amino acid residues. Additionally, CD enzyme mimetics are developed and used as catalysts in traditional artificial enzymes as well as nanozymes, making the application of CDs no longer limited to GHs. This review concerns the regulation of GHs catalysis by CDs, and gives insights into research on interactions between enzymes and ligands.
Original languageEnglish
Article number108326
JournalBiotechnology Advances
Number of pages12
Publication statusPublished - 2024


  • Cyclodextrins
  • Starch-active enzymes
  • Ligands
  • Interactions
  • Regulation of reaction


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