Programmed survival of soil bacteria

Lars Bogø Jensen; Søren Molin; Claus Sternberg; J.L. Ramos

Programmed survival of soil bacteria

Lars Bogø Jensen
, Søren Molin
, Claus Sternberg
, J.L. Ramos

National Food Institute

Institute of Space Sciences

Research output: Contribution to conference › Paper › Research › peer-review

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Abstract

Biological containment systems have been developed for Pseudomonas putida
and related soil bacteria. The systems are based on combinations of lethal genes
and regulated gene expression. Two types of killing function have been
employed: 1) A membrane protein interfering with the membrane potential (geJ).
and 2) a nuclease attacking nucleic acids intracellularly. The efficacy of these
lethal genes has been assessed in model constructions with a synthetic lac promoter.
By combination with the regulatory pathway of the TOL genes. a system
was designed which allows bacterial growth in the presence of aromatics but
results in suicide after degradation of the compound. TIme dependent induction of
suicide is under conslrUction.

Original language	English
Publication date	1992
Number of pages	4
Publication status	Published - 1992
Event	6th International Symposium on Microbial Ecology - Barcelona, Spain Duration: 6 Sept 1992 → 11 Sept 1992 Conference number: 6

Conference

Conference	6th International Symposium on Microbial Ecology
Number	6
Country/Territory	Spain
City	Barcelona
Period	06/09/1992 → 11/09/1992

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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title = "Programmed survival of soil bacteria",

abstract = "Biological containment systems have been developed for Pseudomonas putida and related soil bacteria. The systems are based on combinations of lethal genes and regulated gene expression. Two types of killing function have been employed: 1) A membrane protein interfering with the membrane potential (geJ). and 2) a nuclease attacking nucleic acids intracellularly. The efficacy of these lethal genes has been assessed in model constructions with a synthetic lac promoter. By combination with the regulatory pathway of the TOL genes. a system was designed which allows bacterial growth in the presence of aromatics but results in suicide after degradation of the compound. TIme dependent induction of suicide is under conslrUction.",

author = "Jensen, \{Lars Bog{\o}\} and S{\o}ren Molin and Claus Sternberg and J.L. Ramos",

year = "1992",

language = "English",

note = "6th International Symposium on Microbial Ecology, ISME-6 ; Conference date: 06-09-1992 Through 11-09-1992",

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TY - CONF

T1 - Programmed survival of soil bacteria

AU - Jensen, Lars Bogø

AU - Molin, Søren

AU - Sternberg, Claus

AU - Ramos, J.L.

N1 - Conference code: 6

PY - 1992

Y1 - 1992

N2 - Biological containment systems have been developed for Pseudomonas putida and related soil bacteria. The systems are based on combinations of lethal genes and regulated gene expression. Two types of killing function have been employed: 1) A membrane protein interfering with the membrane potential (geJ). and 2) a nuclease attacking nucleic acids intracellularly. The efficacy of these lethal genes has been assessed in model constructions with a synthetic lac promoter. By combination with the regulatory pathway of the TOL genes. a system was designed which allows bacterial growth in the presence of aromatics but results in suicide after degradation of the compound. TIme dependent induction of suicide is under conslrUction.

AB - Biological containment systems have been developed for Pseudomonas putida and related soil bacteria. The systems are based on combinations of lethal genes and regulated gene expression. Two types of killing function have been employed: 1) A membrane protein interfering with the membrane potential (geJ). and 2) a nuclease attacking nucleic acids intracellularly. The efficacy of these lethal genes has been assessed in model constructions with a synthetic lac promoter. By combination with the regulatory pathway of the TOL genes. a system was designed which allows bacterial growth in the presence of aromatics but results in suicide after degradation of the compound. TIme dependent induction of suicide is under conslrUction.

M3 - Paper

T2 - 6th International Symposium on Microbial Ecology

Y2 - 6 September 1992 through 11 September 1992

ER -

Programmed survival of soil bacteria

Abstract

Conference

UN SDGs

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