Programmed cell death: The life ambition of the barley aleurone layer

Christina Mark, Kinga Zor, Arto Heiskanen, Martin Dufva, Jenny Emnéus, Christine Finnie

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    Abstract

    We have developed a 24-well multiplate tissue culture system with electrochemical and optical detection techniques for cultivation of immobilised barley aleurone layers. We have applied the system for the purpose of studying the underlying mechanisms of programmed cell death (PCD) in plants.
    We have optimised an electrochemical, intracellular, whole-cell redox activity assay [1] that probes the NAD(P):NAD(P)H ratio via a double-mediator system. Experiments show that redox activity changes depend on phytohormone activation or inactivation of aleurone layer metabolism and subsequent PCD.
    We have successfully applied a fluorescent double-probe system [2] to detect PCD to ensure that our redox activity data match with known responses of barley aleurone layers to phytohormones.
    We have also used the system for transformation of barley aleurone cells with α-amylase-GFP constructs for the purpose of studying the timing of α-amylase production in relation to PCD. These studies will be combined with activity assays and quantitative proteomics studies of α-amylase and other target enzymes.
    Original languageEnglish
    Publication date2014
    Publication statusPublished - 2014
    Event1st INPPO World Congress on Plant Proteomics: Methodology to Biology - Universität Hamburg, Hamburg, Germany
    Duration: 31 Aug 20144 Sep 2014
    Conference number: 1
    http://www.inppo2014.uni-hamburg.de/

    Conference

    Conference1st INPPO World Congress on Plant Proteomics
    Number1
    LocationUniversität Hamburg
    CountryGermany
    CityHamburg
    Period31/08/201404/09/2014
    Internet address

    Cite this

    Mark, C., Zor, K., Heiskanen, A., Dufva, M., Emnéus, J., & Finnie, C. (2014). Programmed cell death: The life ambition of the barley aleurone layer. Abstract from 1st INPPO World Congress on Plant Proteomics, Hamburg, Germany.