Activities per year
We have optimised an electrochemical, intracellular, whole-cell redox activity assay  that probes the NAD(P):NAD(P)H ratio via a double-mediator system. Experiments show that redox activity changes depend on phytohormone activation or inactivation of aleurone layer metabolism and subsequent PCD.
We have successfully applied a fluorescent double-probe system  to detect PCD to ensure that our redox activity data match with known responses of barley aleurone layers to phytohormones.
We have also used the system for transformation of barley aleurone cells with α-amylase-GFP constructs for the purpose of studying the timing of α-amylase production in relation to PCD. These studies will be combined with activity assays and quantitative proteomics studies of α-amylase and other target enzymes.
|Publication status||Published - 2014|
|Event||1st INPPO World Congress on Plant Proteomics: Methodology to Biology - Universität Hamburg, Hamburg, Germany|
Duration: 31 Aug 2014 → 4 Sep 2014
Conference number: 1
|Conference||1st INPPO World Congress on Plant Proteomics|
|Period||31/08/2014 → 04/09/2014|
FingerprintDive into the research topics of 'Programmed cell death: The life ambition of the barley aleurone layer'. Together they form a unique fingerprint.
- 1 Conference presentations
Christina Mark (Speaker)31 Aug 2014 → 4 Sep 2014
Activity: Talks and presentations › Conference presentationsFile