Profiling the effect of nafcillin on HA-MRSA D712 using bacteriological and physiological media

Akanksha Rajput; Saugat Poudel; Hannah Tsunemoto; Michael Meehan; Richard Szubin; Connor A. Olson; Anne Lamsa; Yara Seif; Nicholas Dillon; Alison Vrbanac; Joseph Sugie; Samira Dahesh; Jonathan M. Monk; Pieter C. Dorrestein; Rob Knight; Victor Nizet; Bernhard O. Palsson; Adam M. Feist

doi:10.1038/s41597-019-0331-z

Profiling the effect of nafcillin on HA-MRSA D712 using bacteriological and physiological media

Akanksha Rajput, Saugat Poudel, Hannah Tsunemoto, Michael Meehan, Richard Szubin, Connor A. Olson, Anne Lamsa, Yara Seif, Nicholas Dillon, Alison Vrbanac, Joseph Sugie, Samira Dahesh, Jonathan M. Monk, Pieter C. Dorrestein, Rob Knight, Victor Nizet, Bernhard O. Palsson, Adam M. Feist

Novo Nordisk Foundation Center for Biosustainability

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Staphylococcus aureus strains have been continuously evolving resistance to numerous classes of antibiotics including methicillin, vancomycin, daptomycin and linezolid, compounding the enormous healthcare and economic burden of the pathogen. Cation-adjusted Mueller-Hinton broth (CA-MHB) is the standard bacteriological media for measuring antibiotic susceptibility in the clinical lab, but the use of media that more closely mimic the physiological state of the patient, e.g. mammalian tissue culture media, can in certain circumstances reveal antibiotic activities that may be more predictive of effectiveness in vivo. In the current study, we use both types of media to explore antibiotic resistance phenomena in hospital-acquired USA100 lineage methicillin-resistant, vancomycin-intermediate Staphylococcus aureus (MRSA/VISA) strain D712 via multidimensional high throughput analysis of growth rates, bacterial cytological profiling, RNA sequencing, and exo-metabolomics (HPLC and LC-MS). Here, we share data generated from these assays to shed light on the antibiotic resistance behavior of MRSA/VISA D712 in both bacteriological and physiological media.

Original language	English
Article number	322
Journal	Scientific Data
Volume	6
Issue number	1
ISSN	2052-4463
DOIs	https://doi.org/10.1038/s41597-019-0331-z
Publication status	Published - 2019

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Rajput, A., Poudel, S., Tsunemoto, H., Meehan, M., Szubin, R., Olson, C. A., Lamsa, A., Seif, Y., Dillon, N., Vrbanac, A., Sugie, J., Dahesh, S., Monk, J. M., Dorrestein, P. C., Knight, R., Nizet, V., Palsson, B. O., & Feist, A. M. (2019). Profiling the effect of nafcillin on HA-MRSA D712 using bacteriological and physiological media. Scientific Data, 6(1), [322]. https://doi.org/10.1038/s41597-019-0331-z

Rajput, Akanksha ; Poudel, Saugat ; Tsunemoto, Hannah ; Meehan, Michael ; Szubin, Richard ; Olson, Connor A. ; Lamsa, Anne ; Seif, Yara ; Dillon, Nicholas ; Vrbanac, Alison ; Sugie, Joseph ; Dahesh, Samira ; Monk, Jonathan M. ; Dorrestein, Pieter C. ; Knight, Rob ; Nizet, Victor ; Palsson, Bernhard O. ; Feist, Adam M. / Profiling the effect of nafcillin on HA-MRSA D712 using bacteriological and physiological media. In: Scientific Data. 2019 ; Vol. 6, No. 1.

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title = "Profiling the effect of nafcillin on HA-MRSA D712 using bacteriological and physiological media",

abstract = "Staphylococcus aureus strains have been continuously evolving resistance to numerous classes of antibiotics including methicillin, vancomycin, daptomycin and linezolid, compounding the enormous healthcare and economic burden of the pathogen. Cation-adjusted Mueller-Hinton broth (CA-MHB) is the standard bacteriological media for measuring antibiotic susceptibility in the clinical lab, but the use of media that more closely mimic the physiological state of the patient, e.g. mammalian tissue culture media, can in certain circumstances reveal antibiotic activities that may be more predictive of effectiveness in vivo. In the current study, we use both types of media to explore antibiotic resistance phenomena in hospital-acquired USA100 lineage methicillin-resistant, vancomycin-intermediate Staphylococcus aureus (MRSA/VISA) strain D712 via multidimensional high throughput analysis of growth rates, bacterial cytological profiling, RNA sequencing, and exo-metabolomics (HPLC and LC-MS). Here, we share data generated from these assays to shed light on the antibiotic resistance behavior of MRSA/VISA D712 in both bacteriological and physiological media.",

author = "Akanksha Rajput and Saugat Poudel and Hannah Tsunemoto and Michael Meehan and Richard Szubin and Olson, {Connor A.} and Anne Lamsa and Yara Seif and Nicholas Dillon and Alison Vrbanac and Joseph Sugie and Samira Dahesh and Monk, {Jonathan M.} and Dorrestein, {Pieter C.} and Rob Knight and Victor Nizet and Palsson, {Bernhard O.} and Feist, {Adam M.}",

year = "2019",

doi = "10.1038/s41597-019-0331-z",

language = "English",

volume = "6",

journal = "Scientific Data",

issn = "2052-4463",

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Rajput, A, Poudel, S, Tsunemoto, H, Meehan, M, Szubin, R, Olson, CA, Lamsa, A, Seif, Y, Dillon, N, Vrbanac, A, Sugie, J, Dahesh, S, Monk, JM, Dorrestein, PC, Knight, R, Nizet, V, Palsson, BO & Feist, AM 2019, 'Profiling the effect of nafcillin on HA-MRSA D712 using bacteriological and physiological media', Scientific Data, vol. 6, no. 1, 322. https://doi.org/10.1038/s41597-019-0331-z

Profiling the effect of nafcillin on HA-MRSA D712 using bacteriological and physiological media. / Rajput, Akanksha; Poudel, Saugat; Tsunemoto, Hannah; Meehan, Michael; Szubin, Richard; Olson, Connor A.; Lamsa, Anne; Seif, Yara; Dillon, Nicholas; Vrbanac, Alison; Sugie, Joseph; Dahesh, Samira; Monk, Jonathan M.; Dorrestein, Pieter C.; Knight, Rob; Nizet, Victor; Palsson, Bernhard O.; Feist, Adam M.

In: Scientific Data, Vol. 6, No. 1, 322, 2019.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Profiling the effect of nafcillin on HA-MRSA D712 using bacteriological and physiological media

AU - Rajput, Akanksha

AU - Poudel, Saugat

AU - Tsunemoto, Hannah

AU - Meehan, Michael

AU - Szubin, Richard

AU - Olson, Connor A.

AU - Lamsa, Anne

AU - Seif, Yara

AU - Dillon, Nicholas

AU - Vrbanac, Alison

AU - Sugie, Joseph

AU - Dahesh, Samira

AU - Monk, Jonathan M.

AU - Dorrestein, Pieter C.

AU - Knight, Rob

AU - Nizet, Victor

AU - Palsson, Bernhard O.

AU - Feist, Adam M.

PY - 2019

Y1 - 2019

N2 - Staphylococcus aureus strains have been continuously evolving resistance to numerous classes of antibiotics including methicillin, vancomycin, daptomycin and linezolid, compounding the enormous healthcare and economic burden of the pathogen. Cation-adjusted Mueller-Hinton broth (CA-MHB) is the standard bacteriological media for measuring antibiotic susceptibility in the clinical lab, but the use of media that more closely mimic the physiological state of the patient, e.g. mammalian tissue culture media, can in certain circumstances reveal antibiotic activities that may be more predictive of effectiveness in vivo. In the current study, we use both types of media to explore antibiotic resistance phenomena in hospital-acquired USA100 lineage methicillin-resistant, vancomycin-intermediate Staphylococcus aureus (MRSA/VISA) strain D712 via multidimensional high throughput analysis of growth rates, bacterial cytological profiling, RNA sequencing, and exo-metabolomics (HPLC and LC-MS). Here, we share data generated from these assays to shed light on the antibiotic resistance behavior of MRSA/VISA D712 in both bacteriological and physiological media.

AB - Staphylococcus aureus strains have been continuously evolving resistance to numerous classes of antibiotics including methicillin, vancomycin, daptomycin and linezolid, compounding the enormous healthcare and economic burden of the pathogen. Cation-adjusted Mueller-Hinton broth (CA-MHB) is the standard bacteriological media for measuring antibiotic susceptibility in the clinical lab, but the use of media that more closely mimic the physiological state of the patient, e.g. mammalian tissue culture media, can in certain circumstances reveal antibiotic activities that may be more predictive of effectiveness in vivo. In the current study, we use both types of media to explore antibiotic resistance phenomena in hospital-acquired USA100 lineage methicillin-resistant, vancomycin-intermediate Staphylococcus aureus (MRSA/VISA) strain D712 via multidimensional high throughput analysis of growth rates, bacterial cytological profiling, RNA sequencing, and exo-metabolomics (HPLC and LC-MS). Here, we share data generated from these assays to shed light on the antibiotic resistance behavior of MRSA/VISA D712 in both bacteriological and physiological media.

U2 - 10.1038/s41597-019-0331-z

DO - 10.1038/s41597-019-0331-z

M3 - Journal article

C2 - 31848353

VL - 6

JO - Scientific Data

JF - Scientific Data

SN - 2052-4463

IS - 1

M1 - 322

ER -