TY - JOUR
T1 - Profiling the effect of nafcillin on HA-MRSA D712 using bacteriological and physiological media
AU - Rajput, Akanksha
AU - Poudel, Saugat
AU - Tsunemoto, Hannah
AU - Meehan, Michael
AU - Szubin, Richard
AU - Olson, Connor A.
AU - Lamsa, Anne
AU - Seif, Yara
AU - Dillon, Nicholas
AU - Vrbanac, Alison
AU - Sugie, Joseph
AU - Dahesh, Samira
AU - Monk, Jonathan M.
AU - Dorrestein, Pieter C.
AU - Knight, Rob
AU - Nizet, Victor
AU - Palsson, Bernhard O.
AU - Feist, Adam M.
PY - 2019
Y1 - 2019
N2 - Staphylococcus aureus strains have been continuously evolving resistance to numerous classes of antibiotics including methicillin, vancomycin, daptomycin and linezolid, compounding the enormous healthcare and economic burden of the pathogen. Cation-adjusted Mueller-Hinton broth (CA-MHB) is the standard bacteriological media for measuring antibiotic susceptibility in the clinical lab, but the use of media that more closely mimic the physiological state of the patient, e.g. mammalian tissue culture media, can in certain circumstances reveal antibiotic activities that may be more predictive of effectiveness in vivo. In the current study, we use both types of media to explore antibiotic resistance phenomena in hospital-acquired USA100 lineage methicillin-resistant, vancomycin-intermediate Staphylococcus aureus (MRSA/VISA) strain D712 via multidimensional high throughput analysis of growth rates, bacterial cytological profiling, RNA sequencing, and exo-metabolomics (HPLC and LC-MS). Here, we share data generated from these assays to shed light on the antibiotic resistance behavior of MRSA/VISA D712 in both bacteriological and physiological media.
AB - Staphylococcus aureus strains have been continuously evolving resistance to numerous classes of antibiotics including methicillin, vancomycin, daptomycin and linezolid, compounding the enormous healthcare and economic burden of the pathogen. Cation-adjusted Mueller-Hinton broth (CA-MHB) is the standard bacteriological media for measuring antibiotic susceptibility in the clinical lab, but the use of media that more closely mimic the physiological state of the patient, e.g. mammalian tissue culture media, can in certain circumstances reveal antibiotic activities that may be more predictive of effectiveness in vivo. In the current study, we use both types of media to explore antibiotic resistance phenomena in hospital-acquired USA100 lineage methicillin-resistant, vancomycin-intermediate Staphylococcus aureus (MRSA/VISA) strain D712 via multidimensional high throughput analysis of growth rates, bacterial cytological profiling, RNA sequencing, and exo-metabolomics (HPLC and LC-MS). Here, we share data generated from these assays to shed light on the antibiotic resistance behavior of MRSA/VISA D712 in both bacteriological and physiological media.
U2 - 10.1038/s41597-019-0331-z
DO - 10.1038/s41597-019-0331-z
M3 - Journal article
C2 - 31848353
SN - 2052-4463
VL - 6
JO - Scientific Data
JF - Scientific Data
IS - 1
M1 - 322
ER -