Profiling of Substrates for Zinc‐dependent Lysine Deacylase Enzymes: HDAC3 Exhibits Decrotonylase Activity In Vitro

Andreas Stahl Madsen, Christian Adam Olsen

Research output: Contribution to journalJournal articleResearchpeer-review


Systematic screening of the activities of the eleven human zinc-dependent lysine deacylases against a series of fluorogenic substrates (see scheme) as well as kinetic evaluation revealed substrates for screenings of histone deacetylases HDAC10 and HDAC11 at reasonably low enzyme concentrations. Furthermore, HDAC3 in complex with nuclear receptor corepressor 1 (HDAC3–NCoR1) was shown to harbor decrotonylase activity in vitro.
Original languageEnglish
JournalAngewandte Chemie International Edition
Issue number36
Pages (from-to)9083-9087
Publication statusPublished - 2012


  • Enzymes
  • Epigenetics
  • Histone deacetylases
  • Inhibitors
  • Posttranslational modifications

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