Profiling of Indigenous Microbial Community Dynamics and Metabolic Activity During Enrichment in Molasses-Supplemented Crude Oil-Brine Mixtures for Improved Understanding of Microbial Enhanced Oil Recovery

Amalia Yunita Halim, Dorthe Skou Pedersen, Sidsel Marie Nielsen, Anna Eliasson Lantz

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Anaerobic incubations using crude oil and brine from a North Sea reservoir were conducted to gain increased understanding of indigenous microbial community development, metabolite production, and the effects on the oil–brine system after addition of a complex carbon source, molasses, with or without nitrate to boost microbial growth. Growth of the indigenous microbes was stimulated by addition of molasses. Pyrosequencing showed that specifically Anaerobaculum, Petrotoga, and Methanothermococcus were enriched. Addition of nitrate favored the growth of Petrotoga over Anaerobaculum. The microbial growth caused changes in the crude oil–brine system: formation of oil emulsions, and reduction of interfacial tension (IFT). Reduction in IFT was associated with microbes being present at the oil–brine interphase. These findings suggest that stimulation of indigenous microbial growth by addition of molasses has potential as microbial enhanced oil recovery (MEOR) strategy in North Sea oil reservoirs.
Original languageEnglish
JournalApplied Biochemistry and Biotechnology
Volume176
Issue number4
Pages (from-to)1012-1028
Number of pages17
ISSN0273-2289
DOIs
Publication statusPublished - 2015

Keywords

  • Anaerobic
  • MEOR
  • Microbial enrichment
  • Oil emulsion
  • Pyrosequencing

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