Profiling of carbohydrate mixtures at unprecedented resolution using high-precision H-1-C-13 chemical shift measurements and a reference library

Bent Ole Petersen, Ole Hindsgaul, Sebastian Meier

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Complex mixtures of carbohydrates pose distinct challenges in routine and high-throughput analysis, so that only a few carbohydrate components are routinely resolved and identified in biofluids, extracts, foods and other complex mixtures. Here, we conduct precise measurements of H-1 and C-13 anomeric chemical shifts to construct a reference library of specific carbohydrate signals with high-resolution two-dimensional H-1-C-13 NMR spectra. High-resolution multidimensional NMR spectra largely abolish resolution problems in carbohydrate analysis with state-of-the-art instrumentation. Accurate measurements of anomeric H-1-C-13 chemical shifts at parts per billion precisions permit robust carbohydrate identification using a very limited number of instrument-independent reference values.
Original languageEnglish
JournalThe Analyst
Volume139
Issue number2
Pages (from-to)401-406
Number of pages6
ISSN0003-2654
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • Carbohydrates
  • Magnetic Resonance Spectroscopy
  • Reference Standards
  • Solubility
  • CARBOHYDRATES
  • NMR
  • NUCLEAR MAGNETIC RESONANCE
  • Chemistry and biochemistry
  • Food sciences
  • CHEMISTRY,
  • NMR-SPECTROSCOPY
  • OLIGOSACCHARIDES
  • SPECTRA
  • HONEYS

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