Complex mixtures of carbohydrates pose distinct challenges in routine and high-throughput analysis, so that only a few carbohydrate components are routinely resolved and identified in biofluids, extracts, foods and other complex mixtures. Here, we conduct precise measurements of H-1 and C-13 anomeric chemical shifts to construct a reference library of specific carbohydrate signals with high-resolution two-dimensional H-1-C-13 NMR spectra. High-resolution multidimensional NMR spectra largely abolish resolution problems in carbohydrate analysis with state-of-the-art instrumentation. Accurate measurements of anomeric H-1-C-13 chemical shifts at parts per billion precisions permit robust carbohydrate identification using a very limited number of instrument-independent reference values.
- Magnetic Resonance Spectroscopy
- Reference Standards
- NUCLEAR MAGNETIC RESONANCE
- Chemistry and biochemistry
- Food sciences
Petersen, B. O., Hindsgaul, O., & Meier, S. (2014). Profiling of carbohydrate mixtures at unprecedented resolution using high-precision H-1-C-13 chemical shift measurements and a reference library. The Analyst, 139(2), 401-406. https://doi.org/10.1039/c3an01922e