Profile of proinflammatory response in salmonellosis depends on serotype of the pathogen

With the use of enzyme-linked immunosorbent assay (ELISA), we determined the levels of IL-1β, IL-6, IL-10, IL-17А, TNFα, TGFβ, and IL-8 in the blood of patients with salmonellosis caused by Salmonella enterica serovar Typhimurium (acute and chronic course of disease) and S. enterica serovar Enteritidis (acute course of disease) as well as in a healthy subjects (control). We found a statistically significant increase in proinflammatory and anti-inflammatory cytokines in all three groups of patients compared to control. In the acute form of disease, the levels of IL-1β, IL-17А and IL-8 in the blood of patients significantly differed depending on salmonella serotype. In particular, IL-1β was higher in patients infected with S. typhimurium, while the levels of IL-17А and IL-8 were higher in patients infected with S.enteritidis. It was revealed that S. typhimurium caused both acute and chronic course of disease, whereas S. enteritidis – only the acute form. The results of discriminant function analysis with the datasets of four studied groups suggested that the profile of proinflammatory and anti-inflammatory cytokines in salmonellas is in a significant degree conditioned by the serotype of the causative agent.