Abstract
| Original language | English |
|---|---|
| Article number | 4071 |
| Journal | Nature Communications |
| Volume | 10 |
| ISSN | 2041-1723 |
| DOIs | |
| Publication status | Published - 2019 |
Cite this
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Production of zosteric acid and other sulfated phenolic biochemicals in microbial cell factories. / Jendresen, Christian Bille; Nielsen, Alex Toftgaard.
In: Nature Communications, Vol. 10, 4071, 2019.Research output: Contribution to journal › Journal article › Research › peer-review
TY - JOUR
T1 - Production of zosteric acid and other sulfated phenolic biochemicals in microbial cell factories
AU - Jendresen, Christian Bille
AU - Nielsen, Alex Toftgaard
PY - 2019
Y1 - 2019
N2 - Biological production and application of a range of organic compounds is hindered by their limited solubility and toxicity. This work describes a process for functionalization of phenolic compounds that increases solubility and decreases toxicity. We achieve this by screening a wide range of sulfotransferases for their activity towards a range of compounds, including the antioxidant resveratrol. We demonstrate how to engineer cell factories for efficiently creating sulfate esters of phenolic compounds through the use of sulfotransferases and by optimization of sulfate uptake and sulfate nucleotide pathways leading to the 3′-phosphoadenosine 5′-phosphosulfate precursor (PAPS). As an example we produce the antifouling agent zosteric acid, which is the sulfate ester of p-coumaric acid, reaching a titer of 5 g L−1 in fed-batch fermentation. The described approach enables production of sulfate esters that are expected to provide new properties and functionalities to a wide range of application areas.
AB - Biological production and application of a range of organic compounds is hindered by their limited solubility and toxicity. This work describes a process for functionalization of phenolic compounds that increases solubility and decreases toxicity. We achieve this by screening a wide range of sulfotransferases for their activity towards a range of compounds, including the antioxidant resveratrol. We demonstrate how to engineer cell factories for efficiently creating sulfate esters of phenolic compounds through the use of sulfotransferases and by optimization of sulfate uptake and sulfate nucleotide pathways leading to the 3′-phosphoadenosine 5′-phosphosulfate precursor (PAPS). As an example we produce the antifouling agent zosteric acid, which is the sulfate ester of p-coumaric acid, reaching a titer of 5 g L−1 in fed-batch fermentation. The described approach enables production of sulfate esters that are expected to provide new properties and functionalities to a wide range of application areas.
U2 - 10.1038/s41467-019-12022-x
DO - 10.1038/s41467-019-12022-x
M3 - Journal article
VL - 10
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
M1 - 4071
ER -