Production of the artemisinin precursor amorpha-4,11-diene by engineered Saccharomyces cerevisiae

Ann-Louise Lindahl, Mikael Emil Olsson, Per Mercke, Örjan Tollbom, jenny Schelin, Maria Brodelius, Peter E. Brodelius

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The gene encoding for amorpha-4,11-diene synthase from Artemisia annua was transformed into yeast Saccharomyces cerevisiae in two fundamentally different ways. First, the gene was subcloned into the galactose-inducible, high-copy number yeast expression vector pYeDP60 and used to transform the Saccharomyces cerevisiae strain CENÆPK113-5D. Secondly, amorpha-4,11-diene synthase gene, regulated by the same promoter, was introduced into the yeast genome by homologous recombination. In protein extracts from galactose-induced yeast cells, a higher activity was observed for yeast expressing the enzyme from the plasmid. The genome-transformed yeast grows at the same rate as wild-type yeast while plasmidcarrying yeast grows somewhat slower than the wild-type yeast. The plasmid and genome-transformed yeasts produced 600 and 100 lg/l of the artemisinin precursor amorpha-4,11-diene, respectively, during 16- days’ batch cultivation.
Original languageEnglish
JournalBiotechnology Letters
Volume28
Pages (from-to)571-580
ISSN0141-5492
DOIs
Publication statusPublished - 2006
Externally publishedYes

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