Production of the antimicrobial compound tetrabromopyrrole and the Pseudomonas quinolone system precursor, 2‑heptyl‑4‑quinolone, by a novel marine species Pseudoalteromonas galatheae sp. nov.

Sara Skøtt Paulsen, Thomas Isbrandt Petersen, Markus Kirkegaard, Yannick Buijs, Mikael Lenz Strube, Eva Sonnenschein, Thomas Ostenfeld Larsen, Lone Gram*

*Corresponding author for this work

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Abstract

Novel antimicrobials are urgently needed due to the rapid spread of antibiotic resistant bacteria. In a genome-wide analysis of Pseudoalteromonas strains, one strain (S4498) was noticed due to its potent antibiotic activity. It did not produce the yellow antimicrobial pigment bromoalterochromide, which was produced by several related type strains with which it shared less than 95% average nucleotide identity. Also, it produced a sweet-smelling volatile not observed from other strains. Mining the genome of strain S4498 using the secondary metabolite prediction tool antiSMASH led to eight biosynthetic gene clusters with no homology to known compounds, and synteny analyses revealed that the yellow pigment bromoalterochromide was likely lost during evolution. Metabolome profling of strain S4498 using HPLC-HRMS analyses revealed marked diferences to the type strains. In particular, a series of quinolones known as pseudanes were identifed and verifed by NMR. The characteristic odor of the strain was linked to the pseudanes. The highly halogenated compound tetrabromopyrrole was detected as the major antibacterial component by bioassay-guided fractionation. Taken together, the polyphasic analysis demonstrates that strain S4498 belongs to a novel species within the genus Pseudoalteromonas, and we propose the name Pseudoalteromonas galatheae sp. nov. (type strain S4498T =NCIMB 15250T =LMG 31599T).
Original languageEnglish
Article number21630
JournalScientific Reports
Volume10
Number of pages13
ISSN2045-2322
DOIs
Publication statusPublished - 2020

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