Production of L-lysine on different silage juices using genetically engineered Corynebacterium glutamicum

Andreas Neuner, Ines Wagner, Tim Sieker, Roland Ulber, Konstantin Schneider, Susanne Peifer, Elmar Heinzle

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Corynebacterium glutamicum, the best established industrial producer organism for lysine was genetically modified to allow the production of lysine on grass and corn silages. The resulting strain C. glutamicum lysCfbr dldPsod pycPsod malEPsod fbpPsod gapXPsod was based on earlier work (Neuner and Heinzle, 2011). That mutant carries a point mutation in the aspartokinase (lysC) regulatory subunit gene as well as overexpression of d-lactate dehydrogenase (dld), pyruvate carboxylase (pyc) and malic enzyme (malE) using the strong Psod promoter. Here, we additionally overexpressed fructose 1,6-bisphosphatase (fbp) and glyceraldehyde 3-phosphate dehydrogenase (gapX) using the same promoter. The resulting strain grew readily on grass and corn silages with a specific growth rate of 0.35 h-1 and lysine carbon yields of approximately 90 C-mmol (C-mol)-1. Lysine yields were hardly affected by oxygen limitation whereas linear growth was observed under oxygen limiting conditions. Overall, this strain seems very robust with respect to the composition of silage utilizing all quantified low molecular weight substrates, e. g. lactate, glucose, fructose, maltose, quinate, fumarate, glutamate, leucine, isoleucine and alanine.
Original languageEnglish
JournalJournal of Biotechnology
Volume163
Issue number2
Pages (from-to)217-224
Number of pages8
ISSN0168-1656
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Grass silage
  • Corn silage
  • Amino acid production
  • Overexpression
  • Fermentation
  • Oxygen limitation

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