Production of Metabolites

Michael Katz (Inventor), Thomas Durhuus (Inventor), Hans Peter Smits (Inventor), Jochen Förster (Inventor)

Research output: Patent

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Abstract

A recombinant micro-organism such as Saccharomyces cerevisiae which produces and excretes into culture medium a stilbenoid metabolite product when grown under stilbenoid production conditions, which expresses in above native levels a ABC transporter which transports said stilbenoid out of said micro-organism cells to the culture medium. The genome of the Saccharomyces cerevisiae produces an auxotrophic phenotype which is compensated by a plasmid which also expresses one or more of said enzymes constituting said metabolic pathway producing said stilbenoid, an expression product of the plasmid is genetically modified to include a ubiquitination tag sequence. Expression of an enzyme participating in catabolism of phenylalanine by the Ehrlich pathway is optionally reduced compared to its native expression level.
Original languageEnglish
Patent numberWO2011147818
Filing date01/12/2011
CountryInternational Bureau of the World Intellectual Property Organization (WIPO)
Priority date26/05/2010
Priority number1008826.8
Publication statusPublished - 2011

Cite this

Katz, M., Durhuus, T., Smits, H. P., & Förster, J. (2011). Production of Metabolites. (Patent No. WO2011147818).